gen. nov., sp. nov., a nitrogen-fixing bacterium representing a new family in the , fam. nov. Free

Abstract

Five strains representing a novel family within the were isolated from the estuarine grasses and . All strains were facultatively anaerobic, Gram-negative, short, motile, polar monotrichous rods that were mesophilic, oxidase-negative, catalase-positive, had DNA G+C contents of 41.5–44.4 mol% and required seawater salts or NaCl. Growth was observed at pH 3.5–8.0. Polar lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, aminophospholipid, phospholipids and unidentified aminolipids were found in the representative strain S-G2-2. The major menaquinone and ubiquinone were MK-8 (100 %) and Q-8 (93 %), respectively. Predominant fatty acids present were C aldehyde and/or unknown fatty acid 10.9525 (MIDI designation) and/or iso-C I/C 3-OH, Cω7/Cω6, C, C cyclo and Cω7and/or Cω6. The nearly full-length 16S rRNA gene sequences of the strains were very similar (99–100 % similarity), and the strains were identified as members of the same species by DNA–DNA relatedness measurements. 16S rRNA gene sequence analysis revealed that the strains formed a monophyletic lineage within the order . All five strains fixed N. Analysis of partial gene sequences also revealed a monophyletic lineage within the , and the sequences were dissimilar to those of any previously described diazotroph. Differences between the novel strains and other members of the include the inability to produce cytochrome oxidase. The novel strains were metabolically versatile. On the basis of the information described above, the new genus and species gen. nov., sp. nov. are proposed to accommodate the five strains within a new family, fam. nov. The type strain of is S-G2-2 ( = ATCC BAA-1368  = DSM 18577).

Funding
This study was supported by the:
  • NSF (Award MCB-0237854)
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2011-05-01
2024-03-29
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