1887

Abstract

Three short spore chain-forming actinomycete strains were isolated from soil samples collected from subtropical islands in Japan. The cell-wall peptidoglycan of these strains contained -diaminopimelic acid (-Apm), glutamic acid and alanine. The major isoprenoid quinone was MK-9(H), iso-C and 2-OH iso-C were the major cellular fatty acids and phosphatidylethanolamine was a component of the polar lipids. The G+C content of the genomic DNA was 67–69 mol%. Phylogenetic analyses based on the 16S rRNA gene sequences showed that the novel strains consistently formed a monophyletic cluster with . On the basis this polyphasic taxonomical study, it is proposed that the two new isolates represent two novel species: (type strain TT00-43=NBRC 103394=KCTC 19329) and (type strain TT99-32=NBRC 103392=KCTC 19330).

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2010-11-01
2021-10-27
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References

  1. Ezaki T., Hashimoto Y., Takeuchi N., Yamamoto H., Liu S.-L., Miura H., Matsui K., Yabuuchi E. 1988; Simple genetic identification method of viridans group streptococci by colorimetric dot hybridization and quantitative fluorometric hybridization in microdilution wells. J Clin Microbiol 26:1708–1713
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  6. Goodfellow M., Kim S. B., Minnikin D. E., Whitehead D., Zhou Z. H., Mattinson-Rose A. D. 2001; Amycolatopsis sacchari sp. nov., a moderately thermophilic actinomycete isolated from vegetable matter. Int J Syst Evol Microbiol 51:187–193
    [Google Scholar]
  7. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  8. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  9. Hayakawa M., Nonomura H. 1987; Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65:501–509 [CrossRef]
    [Google Scholar]
  10. Hayakawa M., Nonomura H. 1989; A new method for the intensive isolation of actinomycetes from soil. Actinomycetologica 3:95–104 [CrossRef]
    [Google Scholar]
  11. Huang Y., Qi W., Lu Z., Liu Z., Goodfellow M. 2001; Amycolatopsis rubida sp. nov., a new Amycolatopsis species from soil. Int J Syst Evol Microbiol 51:1093–1097 [CrossRef]
    [Google Scholar]
  12. Huang Y., Pasciak M., Liu Z., Xie Q., Gamian A. 2004; Amycolatopsis palatopharyngis sp. nov., a potentially pathogenic actinomycete isolated from a human clinical source. Int J Syst Evol Microbiol 54:359–363 [CrossRef]
    [Google Scholar]
  13. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  14. Kim B., Sahin N., Tan G. Y., Zakrzewska-Czerwinska J., Goodfellow M. 2002; Amycolatopsis eurytherma sp. nov., a thermophilic actinomycete isolated from soil. Int J Syst Evol Microbiol 52:889–894 [CrossRef]
    [Google Scholar]
  15. Labeda D. P., Price N. P., Tan G. Y. A., Goodfellow M., Klenk H.-P. 2010; Emended description of the genus Actinokineospora Hasegawa 1988 and transfer of Amycolatopsis fastidiosa Henssen et al. 1987 as Actinokineospora fastidiosa comb. nov. Int J Syst Evol Microbiol 60:1444–1449 [CrossRef]
    [Google Scholar]
  16. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  17. Lechevalier M. P., Prauser H., Labeda D. P., Ruan J.-S. 1986; Two new genera of nocardioform actinomycetes: Amycolata gen.nov. and Amycolatopsis gen. nov.. Int J Syst Bacteriol 36:29–37 [CrossRef]
    [Google Scholar]
  18. MIDI 2002 MIS operating manual , version 4.5 Newark, Delaware: MIDI, Inc;
    [Google Scholar]
  19. Rayner R. W. 1970 A mycological colour chart Kew: UK. ; Commonwealth Mycological Institute;
    [Google Scholar]
  20. Saintpierre-Bonaccio D., Amir H., Pineau R., Tan G. Y., Goodfellow M. 2005; Amycolatopsis plumensis sp. nov., a novel bioactive actinomycete isolated from a New-Caledonian brown hypermagnesian ultramafic soil. Int J Syst Evol Microbiol 55:2057–2061 [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  23. Seino A., Arai M., Enokita R., Okazaki T., Furuichi A. 1985 Identification manual of actinomycetes Tokyo: The Society for Actinomycetes Japan;
    [Google Scholar]
  24. Swofford D. L. 2002 paup*: phylogenetic analysis using parsimony (*and other methods), version 4.0b10 Sunderland, MA: Sinauer;
    [Google Scholar]
  25. Tamura T., Hatano K. . (; 2001; Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘ Actinoplanes aurantiacus ’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov. . Int J Syst Evol Microbiol 51:2119–2125 [CrossRef]
    [Google Scholar]
  26. Tamura T., Nakagaito Y., Nishii T., Hasegawa T., Stackebrandt E., Yokota A. 1994; A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb.nov. and Couchioplanescaeruleus subsp. azureus subsp. nov.. Int J Syst Bacteriol 44:193–203 [CrossRef]
    [Google Scholar]
  27. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  28. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  29. Tseng M., Yang S. F., Li W. J., Jiang C. L. 2006; Amycolatopsis taiwanensis sp. nov., from soil. Int J Syst Evol Microbiol 56:1811–1815 [CrossRef]
    [Google Scholar]
  30. Yokota A., Tamura T., Hasegawa T., Huang L. H. 1993; Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . Int J Syst Bacteriol 43:805–812 [CrossRef]
    [Google Scholar]
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