1887

Abstract

-like isolates, strains W03-356, W03-357 and W03-359, from three independent water samples from the river Elbe, Germany, were analysed by using a polyphasic approach. Morphological and biochemical characterization revealed that they were Gram-negative, aerobic, non-spore-forming bacilli with a cut glass colony appearance that grew only on -cysteine-supplemented buffered charcoal yeast extract agar. Phylogenetic analysis based on sequence comparisons of the 16S rRNA, macrophage infectivity potentiator (), gyrase subunit A (), ribosomal polymerase B () and RNase P () genes confirmed that the three isolates were distinct from recognized species of the genus . Phenotypic characterization of strain W03-356 based on fatty acid profiles confirmed that it was closely related to ATCC 35304 and ATCC 33152, but distinct from other species of the genus . Serotyping of the isolates showed that they were distinct from all recognized species of the genus . Strains W03-356, W03-357 and W03-359 are thus considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W03-356 (=DSM 19488=NCTC 13409).

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2010-11-01
2019-12-15
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vol. , part 11, pp. 2557 - 2562

List of type strains of species of the genus used for indirect immunofluorescence assays with a rabbit antiserum prepared against strain W03-356 .

List of the 46 strains of the genus for which antisera were prepared and tested against the three new isolates.

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