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Abstract

Two xylan-degrading bacteria, strains MX15-2 and MX21-2, were isolated from soils collected in Nan province, Thailand. Cells were Gram-reaction-positive, facultatively anaerobic, spore-forming and rod-shaped. They contained -diaminopimelic acid in the cell-wall peptidoglycan. The major menaquinone was MK-7. iso-C and anteiso-C were the predominant cellular fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol were the major polar lipids. The genomic DNA G+C contents of strains MX15-2 and MX21-2 were 63.0 and 65.1 mol%, respectively. Phylogenetic analysis using 16S rRNA gene sequences showed that strains MX15-2 and MX21-2 were affiliated with the genus and were closely related to CCUG 47242, with 96.5 and 95.6 % sequence similarity, respectively. The strains could be clearly distinguished from each other and from all known species of the genus based on their physiological and biochemical characteristics as well as their phylogenetic positions and levels of DNA–DNA hybridization. Therefore, these two strains represent novel species of the genus , for which the names sp. nov. (type strain MX15-2 =KCTC 22294 =PCU 309 =TISTR 1891) and sp. nov. (type strain MX21-2 =KCTC 22295 =PCU 310 =TISTR 1892) are proposed.

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2010-12-01
2020-01-24
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vol. , part 12, pp. 2913 - 2917

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Neighbour-joining tree based on 16S rRNA gene sequences showing the phylogenetic relationship between strains MX15-2 and MX21-2 , species of the genus and related taxa. Based on 1000 resamplings, bootstrap percentages above 50% are shown. Bar, 0.01 substitutions per nucleotide position. [PDF]

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Maximum-parsimony tree based on 16S rRNA gene sequences showing the phylogenetic relationship between strains MX15-2 and MX21-2 , species of the genus and related taxa. Based on 1000 replications, bootstrap percentages above 50% are shown. [PDF]

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Maximum-parsimony tree based on 16S rRNA gene sequences showing the phylogenetic relationship between strains MX15-2 and MX21-2 , species of the genus and related taxa. Based on 1000 replications, bootstrap percentages above 50% are shown. [PDF]

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