1887

Abstract

Three strains of Gram-negative, rod-shaped, non-spore-forming bacteria (M 2040, M 1973 and M 1878-SK2), isolated from milk of camels at a camel-milk production farm in the United Arab Emirates, were investigated for their taxonomic allocation. On the basis of 16S rRNA gene sequence similarities, all three strains were shown to belong to the and were most closely related to and (95.1 and 95.2 % sequence similarity to the respective type strains). Diaminopimelic acid was detected as the characteristic peptidoglycan diamino acid. The predominant compound in the polyamine pattern was spermidine, and homospermidine was not detectable. The quinone system was ubiquinone Q-10. The polar lipid profile included the major compounds phosphatidylcholine and diphosphatidylglycerol and moderate amounts of phosphatidylethanolamine, phosphatidylglycerol, an unidentified glycolipid and two unidentified aminolipids. Minor lipids were also detected. The major fatty acid profile, consisting of C cyclo 8 and C 7, with C 3-OH as the major hydroxylated fatty acid, was similar to those of the genus . The results of DNA–DNA hybridization experiments and physiological and biochemical tests allowed both genotypic and phenotypic differentiation of the isolates from described species. Isolates M 2040, M 1973 and M 1878-SK2 were closely related on the basis of DNA–DNA reassociation and therefore represent a single novel species. In summary, low 16S rRNA gene sequence similarities of 95 % with and marked differences in polar lipid profiles as well as in polyamine patterns support the description of a novel genus and species to accommodate these strains, for which the name gen. nov., sp. nov. is proposed. The type strain of is M 2040 (=CCUG 58638 =CCM 7696).

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2010-10-01
2024-10-06
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References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [CrossRef]
    [Google Scholar]
  2. Auling G., Busse H.-J., Egli T., El-Banna T., Stackebrandt E. 1993; Description of the Gram-negative, obligately aerobic, nitrilotriacetate (NTA)-utilizing bacteria as Chelatobacter heintzii , gen. nov., sp. nov., and Chelatococcus asaccharovorans , gen. nov. sp. nov.. Syst Appl Microbiol 16:104–112
    [Google Scholar]
  3. Busse H.-J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11:1–8 [CrossRef]
    [Google Scholar]
  4. Christensen W. B. 1946; Urea decomposition as a means of differentiating Proteus and paracolon cultures from each other and from Salmonella and Shigella types. J Bacteriol 52:461–466
    [Google Scholar]
  5. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  6. Huber B., Scholz H. C., Kämpfer P., Falsen E., Langer S., Busse H.-J. 2010; Ochrobactrum pituitosum sp. nov., isolated from an industrial environment. Int J Syst Evol Microbiol 60:321–326 [CrossRef]
    [Google Scholar]
  7. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [CrossRef]
    [Google Scholar]
  8. Kämpfer P., Dreyer U., Neef A., Dott W., Busse H.-J. 2003a; Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53:93–97 [CrossRef]
    [Google Scholar]
  9. Kämpfer P., Buczolits S., Albrecht A., Busse H.-J., Stackebrandt E. 2003b; Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov. Int J Syst Evol Microbiol 53:893–896 [CrossRef]
    [Google Scholar]
  10. Kämpfer P., Scholz H. C., Huber B., Falsen E., Busse H.-J. 2007; Ochrobactrum haematophilum sp. nov. and Ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens. Int J Syst Evol Microbiol 57:2513–2518 [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Sessitsch A., Schloter M., Huber B., Busse H.-J., Scholz H. C. 2008; Ochrobactrum rhizosphaerae sp. nov. and Ochrobactrum thiophenivorans sp. nov., isolated from the environment. Int J Syst Evol Microbiol 58:1426–1431 [CrossRef]
    [Google Scholar]
  12. Kumar S., Tamura K., Jakobsen I.-B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  13. Ludwig W., Strunk O., Westram R., Richer L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  14. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  15. Stolz A., Busse H.-J., Kämpfer P. 2007; Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57:572–576 [CrossRef]
    [Google Scholar]
  16. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  17. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [CrossRef]
    [Google Scholar]
  18. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
    [Google Scholar]
  19. Wieser M., Busse H.-J. 2000; Rapid identification of Staphylococcus epidermidis . Int J Syst Evol Microbiol 50:1087–1093 [CrossRef]
    [Google Scholar]
  20. Yoon J.-H., Kang S.-J., Im W.-T., Lee S.-T., Oh T.-K. 2008; Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus . Int J Syst Evol Microbiol 58:2224–2228 [CrossRef]
    [Google Scholar]
  21. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186 [CrossRef]
    [Google Scholar]
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