1887

Abstract

A Gram-stain-negative, motile, aerobic bacterial strain, designated MJ03, was isolated from sewage and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ03 belongs to the family , class , and was related most closely to AMX 19 (97.9 % sequence similarity), R-32729 (97.1 %), L2 (96.9 %), ATCC 13637 (96.8 %) and R-32768 (96.7 %). The G+C content of the genomic DNA of strain MJ03 was 64.7 mol%. The detection of a quinone system with ubiquinone Q-8 as the predominant component and a fatty acid profile with iso-C, iso-C, iso-C, iso-C 9, iso-C 3-OH and iso-C 3-OH as major components supported the affiliation of strain MJ03 to the genus . However, levels of DNA–DNA relatedness between strain MJ03 and the type strains of five closely related species of the genus ranged from 11 to 34 %, showing clearly that the isolate represents a novel genospecies. Strain MJ03 could be differentiated clearly from its phylogenetic neighbours on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ03 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MJ03 (=KCTC 22451 =JCM 16244).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.017780-0
2011-03-01
2024-11-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/3/598.html?itemId=/content/journal/ijsem/10.1099/ijs.0.017780-0&mimeType=html&fmt=ahah

References

  1. Assih E. A., Ouattara A. S., Thierry S., Cayol J.-L., Labat M., Macarie H. 2002; Stenotrophomonas acidaminiphila sp. nov., a strictly aerobic bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor. Int J Syst Evol Microbiol 52:559–568
    [Google Scholar]
  2. Atlas R. M. 1993 Handbook of Microbiological Media Edited by Parks L. C. Boca Raton, FL: CRC Press;
    [Google Scholar]
  3. Cappuccino J. G., Sherman N. 2002 Microbiology: a Laboratory Manual, 6th edn. Menlo Park, CA: Benjamin/Cummings;
    [Google Scholar]
  4. Collins C. H., Lyne P. M. 1984 Microbiological Methods, 5th edn. London: Butterworth;
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric DNA–DNA hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 2009 phylip (phylogeny inference package), version 3.69. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  9. Finkmann W., Altendorf K., Stackebrandt E., Lipski A. 2000; Characterization of N2O-producing Xanthomonas -like isolates from biofilters as Stenotrophomonas nitritireducens sp.nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov. Int J Syst Evol Microbiol 50:273–282 [CrossRef]
    [Google Scholar]
  10. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology.
  11. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  12. Heylen K., Vanparys B., Peirsegaele F., Lebbe L., De Vos P. 2007; Stenotrophomonas terrae sp. nov. and Stenotrophomonas humi sp. nov., two nitrate-reducing bacteria isolated from soil. Int J Syst Evol Microbiol 57:2056–2061 [CrossRef]
    [Google Scholar]
  13. Hiraishi A., Ueda Y., Ishihara J., Mori T. 1996; Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469 [CrossRef]
    [Google Scholar]
  14. Hsu S. C., Lockwood J. L. 1975; Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 29:422–426
    [Google Scholar]
  15. Kim M. K., Im W.-T., Ohta H., Lee M., Lee S.-T. 2005; Sphingopyxis granuli sp. nov., a beta-glucosidase-producing bacterium in the family Sphingomonadaceae in alpha-4 subclass of the Proteobacteria . J Microbiol 43:152–157
    [Google Scholar]
  16. Kim H.-B., Srinivasan S., Sathiyaraj G., Quan L.-H., Kim S.-H., Bui T. P. N., Liang Z.-Q., Kim Y.-J., Yang D.-C. 2010; Stenotrophomonas ginsengisoli sp. nov., isolated from a ginseng field. Int J Syst Evol Microbiol 60:1522–1526 [CrossRef]
    [Google Scholar]
  17. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  19. Moore D. D., Dowhan D. 1995; Preparation and analysis of DNA. In Current Protocols in Molecular Biology pp 2–11 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Wiley;
    [Google Scholar]
  20. Palleroni N. J., Bradbury J. F. 1993; Stenotrophomonas , a new bacterial genus for Xanthomonas maltophila ; (Hugh 1980; Swings et al. 1983. Int J Syst Bacteriol 43:606–609 [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Sasser M. 1990; Identification of bacteria through fatty acid analysis. In Methods in Phytobacteriology pp 199–204 Edited by Klement Z., Rudolph K., Sands D. C. Budapest: Akademiai Kaido;
    [Google Scholar]
  23. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  24. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C., Nesme X., Rosselló-Mora R, Swings J. other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  25. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  26. Ten L. N., Im W.-T., Kim M.-K., Kang M.-S., Lee S.-T. 2004; Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. J Microbiol Methods 56:375–382 [CrossRef]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  28. Tindall J. B., Sikorski J., Simbert A. R., Krieg R. N. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. pp 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  30. Wolf A., Fritze A., Hagemann M., Berg G. 2002; Stenotrophomonas rhizophila sp. nov., a novel plant-associated bacterium with antifungal properties. Int J Syst Evol Microbiol 52:1937–1944 [CrossRef]
    [Google Scholar]
  31. Yang H.-C., Im W.-T., Kang M. S., Shin D.-Y., Lee S.-T. 2006; Stenotrophomonas koreensis sp. nov., isolated from compost in South Korea. Int J Syst Evol Microbiol 56:81–84 [CrossRef]
    [Google Scholar]
  32. Yoon J.-H., Kang S.-J., Oh H. W., Oh T.-K. 2006; Stenotrophomonas dokdonensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:1363–1367 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.017780-0
Loading
/content/journal/ijsem/10.1099/ijs.0.017780-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error