1887

Abstract

A Gram-stain-negative, motile, aerobic bacterial strain, designated MJ03, was isolated from sewage and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ03 belongs to the family , class , and was related most closely to AMX 19 (97.9 % sequence similarity), R-32729 (97.1 %), L2 (96.9 %), ATCC 13637 (96.8 %) and R-32768 (96.7 %). The G+C content of the genomic DNA of strain MJ03 was 64.7 mol%. The detection of a quinone system with ubiquinone Q-8 as the predominant component and a fatty acid profile with iso-C, iso-C, iso-C, iso-C 9, iso-C 3-OH and iso-C 3-OH as major components supported the affiliation of strain MJ03 to the genus . However, levels of DNA–DNA relatedness between strain MJ03 and the type strains of five closely related species of the genus ranged from 11 to 34 %, showing clearly that the isolate represents a novel genospecies. Strain MJ03 could be differentiated clearly from its phylogenetic neighbours on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ03 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MJ03 (=KCTC 22451 =JCM 16244).

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2011-03-01
2020-01-21
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