1887

Abstract

A novel strictly anaerobic, moderately halophilic and mesophilic bacterium, designated strain SOL3f37, was isolated from a hydrocarbon-polluted soil surrounding a deep petroleum environment located in south Tunisia. Cells of strain SOL3f37 stained Gram-positive and were motile, straight and spore-forming. Strain SOL3f37 had a typical Gram-positive-type cell-wall structure, unlike the thick, multilayered cell wall of its closest relative . The major fatty acids were iso-C (41 %), iso-C 3-OH and/or iso-C dimethyl acetal (21.6 %), iso-C (4.4 %), anteiso-C (3.9 %) and iso-C (2.8 %). Strain SOL3f37 grew between 20 and 48 °C (optimum 40 °C) and at pH 6.2–8.1 (optimum pH 6.9). Strain SOL3f37 required at least 0.5 g NaCl l and grew in the presence of NaCl concentrations up to 150 g l (optimum 40 g l). Yeast extract (2 g l) was required for degradation of pyruvate, fumarate, fructose, glucose and mannitol. Also, strain SOL3f37 grew heterotrophically on yeast extract, peptone and bio-Trypticase, but was unable to grow on Casamino acids. Sulfate, thiosulfate, sulfite, elemental sulfur, fumarate, nitrate and nitrite were not reduced. The DNA G+C content was 30.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SOL3f37 was a member of the family in the order ; strain SOL3f37 was related to members of various genera of the family . It exhibited highest 16S rRNA gene sequence similarity (93.4 %) with 37HS60, 91.8 % with CTT3 and 91.7 % with MV1087. On the basis of genotypic, phenotypic and phylogenetic data, it is suggested that strain SOL3f37 represents a novel species in a new genus. The name gen. nov., sp. nov. is proposed, with SOL3f37 (=DSM 21485 =JCM 15487) as the type strain of .

Keyword(s): DMA, dimethyl acetal
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2011-01-01
2019-10-23
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