1887

Abstract

A Gram-negative, non-motile, non-gliding, strictly aerobic, pale yellow colony-forming, rod-shaped bacterial strain, designated HM0024, was isolated from coastal seawater of the Yellow Sea and subjected to a polyphasic taxonomy study. Based on 16S rRNA gene sequence similarities, strain HM0024 was most closely related to LMG 8329 (94.4 %), LMG 13028 (94.4 %), LMG 23089 (94.3 %) and ATCC 13253 (94.0 %) and shared less than 92 % sequence similarity with other members of the family . Phylogenetic analyses showed that strain HM0024 formed an independent phyletic line of descent within the family . The DNA G+C content of the strain was 29.6 mol% and its major cellular fatty acids (>10 %) were iso-C, iso-C 3-OH, iso-C 7 and summed feature 3 (C 6 and/or C 7). The major isoprenoid quinone was MK-6 and the major polar lipids were phosphatidylethanolamine and several aminolipids. Strain HM0024 was differentiated from phylogenetically related members of the family by having lower DNA G+C content, larger proportions of summed feature 3, anteiso-C and iso-C 3-OH and particular phenotypic characteristics. On the basis of phenotypic and phylogenetic data, strain HM0024 is classified as a representative of a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is HM0024 (=KCTC 22689 =CECT 7503).

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2010-09-01
2020-09-21
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References

  1. Bae S. S., Kwon K. K., Yang S. H., Lee H. S., Kim S. J., Lee J. H. 2007; Flagellimonas eckloniae gen. nov., sp. nov. a mesophilic marine bacterium of the family Flavobacteriaceae , isolated from the rhizosphere of Ecklonia kurome . Int J Syst Evol Microbiol 57:1050–1054 [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y. 2006; An introduction to the family Flavobacteriaceae . In The Prokaryotes: A Handbook on the Biology of Bacteria. , 3rd edn. vol 7 pp 455–480 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
  3. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  4. Bernardet J. F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  5. Bowman J. P., McCammon S. A., Brown J. L., Nichols P. D., McMeekin T. A. 1997; Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from Antarctic lacustrine and sea ice habitats. Int J Syst Bacteriol 47:670–677 [CrossRef]
    [Google Scholar]
  6. Cho J.-C., Giovannoni S. J. 2003; Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α - Proteobacteria . Int J Syst Evol Microbiol 53:1031–1036 [CrossRef]
    [Google Scholar]
  7. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  8. Einen J., Øvreås L. 2006; Flaviramulus basaltis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seafloor basalt. Int J Syst Evol Microbiol 56:2455–2461 [CrossRef]
    [Google Scholar]
  9. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  10. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  11. Khan S. T., Nakagawa Y., Harayama S. 2008; Fulvibacter tottoriensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment. Int J Syst Evol Microbiol 581670–1674 [CrossRef]
    [Google Scholar]
  12. Kim K. K., Kim M. K., Lim J. H., Park H. Y., Lee S. T. 2005; Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov. Int J Syst Evol Microbiol 55:1287–1293 [CrossRef]
    [Google Scholar]
  13. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  14. Macián M. C., Pujalte M. J., Márquez M. C., Ludwig W., Ventosa A., Garay E., Schleifer K. H. 2002; Gelidibacter mesophilus sp. nov., a novel marine bacterium in the family Flavobacteriaceae . Int J Syst Evol Microbiol 52:1325–1329 [CrossRef]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  16. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  17. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Bae K. S. 2005; Bizionia paragorgiae gen. nov., sp. nov. a novel member of the family Flavobacteriaceae isolated from the soft coral Paragorgia arborea . Int J Syst Evol Microbiol 55:375–378 [CrossRef]
    [Google Scholar]
  18. Peterson W. J., Bell T. A., Etchells J. L., Smart W. W. G. Jr 1954; A procedure for demonstrating the presence of carotenoid pigments in yeasts. J Bacteriol 67:708–713
    [Google Scholar]
  19. Romanenko L. A., Uchino M., Frolova G. M., Mikhailov V. V. 2007; Marixanthomonas ophiurae gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a deep-sea brittle star. Int J Syst Evol Microbiol 57:457–462 [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Swofford D. L. 2002 paup*: phylogenetic analysis using parsimony (and other methods), version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  22. Vandamme P., Bernardet J.-F., Segers P., Kersters K., Holmes B. 1994; New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen.nov., Bergeyella gen. nov., and Empedobacter nom. , . rev. Int J Syst Bacteriol 44827–831 [CrossRef]
    [Google Scholar]
  23. Yang S. J., Cho J.-C. 2008; Gaetbulibacter marinus sp. nov., isolated from coastal seawater, and emended description of the genus Gaetbulibacter . Int J Syst Evol Microbiol 58:315–318 [CrossRef]
    [Google Scholar]
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