1887

Abstract

A Gram-negative, non-motile, non-gliding, strictly aerobic, pale yellow colony-forming, rod-shaped bacterial strain, designated HM0024, was isolated from coastal seawater of the Yellow Sea and subjected to a polyphasic taxonomy study. Based on 16S rRNA gene sequence similarities, strain HM0024 was most closely related to LMG 8329 (94.4 %), LMG 13028 (94.4 %), LMG 23089 (94.3 %) and ATCC 13253 (94.0 %) and shared less than 92 % sequence similarity with other members of the family . Phylogenetic analyses showed that strain HM0024 formed an independent phyletic line of descent within the family . The DNA G+C content of the strain was 29.6 mol% and its major cellular fatty acids (>10 %) were iso-C, iso-C 3-OH, iso-C 7 and summed feature 3 (C 6 and/or C 7). The major isoprenoid quinone was MK-6 and the major polar lipids were phosphatidylethanolamine and several aminolipids. Strain HM0024 was differentiated from phylogenetically related members of the family by having lower DNA G+C content, larger proportions of summed feature 3, anteiso-C and iso-C 3-OH and particular phenotypic characteristics. On the basis of phenotypic and phylogenetic data, strain HM0024 is classified as a representative of a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is HM0024 (=KCTC 22689 =CECT 7503).

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2010-09-01
2019-12-11
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References

  1. Bae, S. S., Kwon, K. K., Yang, S. H., Lee, H. S., Kim, S. J. & Lee, J. H. ( 2007; ). Flagellimonas eckloniae gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from the rhizosphere of Ecklonia kurome. Int J Syst Evol Microbiol 57, 1050–1054.[CrossRef]
    [Google Scholar]
  2. Bernardet, J.-F. & Nakagawa, Y. ( 2006; ). An introduction to the family Flavobacteriaceae. In The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 455–480. Edited by Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K. H. & Stackebrandt, E.. New York. : Springer.
    [Google Scholar]
  3. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P. ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef]
    [Google Scholar]
  4. Bernardet, J. F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  5. Bowman, J. P., McCammon, S. A., Brown, J. L., Nichols, P. D. & McMeekin, T. A. ( 1997; ). Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from Antarctic lacustrine and sea ice habitats. Int J Syst Bacteriol 47, 670–677.[CrossRef]
    [Google Scholar]
  6. Cho, J.-C. & Giovannoni, S. J. ( 2003; ). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria. Int J Syst Evol Microbiol 53, 1031–1036.[CrossRef]
    [Google Scholar]
  7. Chun, J., Lee, J. H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  8. Einen, J. & Øvreås, L. ( 2006; ). Flaviramulus basaltis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seafloor basalt. Int J Syst Evol Microbiol 56, 2455–2461.[CrossRef]
    [Google Scholar]
  9. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  10. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  11. Khan, S. T., Nakagawa, Y. & Harayama, S. ( 2008; ). Fulvibacter tottoriensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment. Int J Syst Evol Microbiol 58, 1670–1674.[CrossRef]
    [Google Scholar]
  12. Kim, K. K., Kim, M. K., Lim, J. H., Park, H. Y. & Lee, S. T. ( 2005; ). Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov. Int J Syst Evol Microbiol 55, 1287–1293.[CrossRef]
    [Google Scholar]
  13. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  14. Macián, M. C., Pujalte, M. J., Márquez, M. C., Ludwig, W., Ventosa, A., Garay, E. & Schleifer, K. H. ( 2002; ). Gelidibacter mesophilus sp. nov., a novel marine bacterium in the family Flavobacteriaceae. Int J Syst Evol Microbiol 52, 1325–1329.[CrossRef]
    [Google Scholar]
  15. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  16. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  17. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Frolova, G. M., Mikhailov, V. V. & Bae, K. S. ( 2005; ). Bizionia paragorgiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the soft coral Paragorgia arborea. Int J Syst Evol Microbiol 55, 375–378.[CrossRef]
    [Google Scholar]
  18. Peterson, W. J., Bell, T. A., Etchells, J. L. & Smart, W. W. G., Jr ( 1954; ). A procedure for demonstrating the presence of carotenoid pigments in yeasts. J Bacteriol 67, 708–713.
    [Google Scholar]
  19. Romanenko, L. A., Uchino, M., Frolova, G. M. & Mikhailov, V. V. ( 2007; ). Marixanthomonas ophiurae gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a deep-sea brittle star. Int J Syst Evol Microbiol 57, 457–462.[CrossRef]
    [Google Scholar]
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Swofford, D. L. ( 2002; ). paup*: phylogenetic analysis using parsimony (and other methods), version 4. Sunderland, MA: Sinauer Associates.
  22. Vandamme, P., Bernardet, J.-F., Segers, P., Kersters, K. & Holmes, B. ( 1994; ). New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44, 827–831.[CrossRef]
    [Google Scholar]
  23. Yang, S. J. & Cho, J.-C. ( 2008; ). Gaetbulibacter marinus sp. nov., isolated from coastal seawater, and emended description of the genus Gaetbulibacter. Int J Syst Evol Microbiol 58, 315–318.[CrossRef]
    [Google Scholar]
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