1887

Abstract

A strictly aerobic, Gram-negative, non-pigmented bacterial strain, designated LMEB 39, was isolated from a seawater sample collected from the Yangtze River estuary near the East China Sea and was examined physiologically, chemotaxonomically and phylogenetically. The novel isolate was motile by a single polar flagellum and positive for nitrate reduction and decomposition of casein, gelatin, Tween 20 and Tween 80, but negative for indole production. Chemotaxonomic analysis revealed ubiquinone-8 as the predominant respiratory quinone and phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. The major fatty acids were C 7/iso-C 2-OH, C, C 7, C 3-OH, C 8 and C. The genomic DNA G+C content was 42.3 mol%. Comparative 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus . Strain LMEB 39 exhibited the closest phylogenetic affinity to JCM 12483 (97.4 % sequence similarity). The DNA–DNA reassociation values between strain LMEB 39 and JCM 12483 and DSM 6065 (97.2 % sequence similarity) were 31.7 and 30.3 %, respectively. On the basis of phenotypic and genotypic data, strain LMEB 39 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is LMEB 39 (=CGMCC 1.8499=JCM 15903).

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2010-09-01
2020-01-27
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Transmission electron micrograph of strain LMEB 39 , showing a slightly ovoid rod with a polar flagellum. Bar, 1 µm.

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