
Full text loading...
A strictly aerobic, Gram-negative, non-pigmented bacterial strain, designated LMEB 39T, was isolated from a seawater sample collected from the Yangtze River estuary near the East China Sea and was examined physiologically, chemotaxonomically and phylogenetically. The novel isolate was motile by a single polar flagellum and positive for nitrate reduction and decomposition of casein, gelatin, Tween 20 and Tween 80, but negative for indole production. Chemotaxonomic analysis revealed ubiquinone-8 as the predominant respiratory quinone and phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. The major fatty acids were C16 : 1 ω7c/iso-C15 : 0 2-OH, C16 : 0, C18 : 1 ω7c, C12 : 0 3-OH, C17 : 1 ω8c and C17 : 0. The genomic DNA G+C content was 42.3 mol%. Comparative 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Pseudoalteromonas. Strain LMEB 39T exhibited the closest phylogenetic affinity to Pseudoalteromonas byunsanensis JCM 12483T (97.4 % sequence similarity). The DNA–DNA reassociation values between strain LMEB 39T and P. byunsanensis JCM 12483T and Pseudoalteromonas undina DSM 6065T (97.2 % sequence similarity) were 31.7 and 30.3 %, respectively. On the basis of phenotypic and genotypic data, strain LMEB 39T represents a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas lipolytica sp. nov. is proposed; the type strain is LMEB 39T (=CGMCC 1.8499T=JCM 15903T).
Article metrics loading...
Full text loading...
References
Data & Media loading...
Supplements