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Abstract

A Gram-negative, rod-shaped, non-pigmented, non-spore-forming bacterial strain that was motile by a single polar flagellum, designated A1-9, was isolated from Daqing reservoir in north-east China and its taxonomic position was studied using a polyphasic approach. Strain A1-9 was non-halophilic, strictly aerobic and heterotrophic and lacked carotenoids, internal membranes and genes for photosynthesis ( genes). Strain A1-9 grew at 10–40 °C (optimum, 25–30 °C) and pH 5.5–9.0 (optimum, pH 6.0–6.5) and tolerated up to 1.0 % NaCl (w/v). Neither phototrophic nor fermentative growth was observed. The predominant ubiquinone was Q-10 and the major fatty acid was C 7 (70 %). The DNA G+C content was 64.4 mol% ( ). Phylogenetic analyses based on the 16S rRNA gene sequence revealed that strain A1-9, together with AST4, formed a deep line within the ‘ clade’ of the family and strain A1-9 showed 94.2 % 16S rRNA gene sequence similarity to AST4. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain A1-9 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A1-9 (=CGMCC 1.7029 =NBRC 104254).

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2010-09-01
2019-10-14
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vol. , part 9, pp. 2027 - 2031

Transmission electron micrograph of cells of strain A1-9 grown on YP agar for 2 days at 30 °C.



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