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Abstract

A bacterial strain, designated P15, was isolated from the soil of an open hexachlorocyclohexane dumpsite. Comparative sequence analysis showed that strain P15 displayed high 16S rRNA gene sequence similarities (94.4–97.2 %) with members of the genus . The isolate was most closely related to AMX 26B (97.2 % 16S rRNA gene sequence similarity) and 12-3 (97.2 %). DNA–DNA relatedness studies showed unambiguously that strain P15 represented a novel species that was separate from . DSM 17121 (7.7 %) and . DSM 17109 (9.4 %). The predominant cellular fatty acids of strain P15 were iso-C (21.4 %), iso-C (16.1 %), summed feature 9 (comprising iso-Cω9 and/or 10-methyl C; 14.9 %), iso-C 3-OH (8.3 %) and iso-C (7.0 %). The polar lipid profile of strain P15 showed the presence of large amounts of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol in addition to unknown glycolipids, phospholipids and an amino-group-containing polar lipid. Ubiquinone 8 was found as the major quinone. The polyamine profile showed the presence of spermidine. The DNA G+C content was 62.9±2 mol%. Strain P15 is described as representing a new member of the genus , for which the name sp. nov. is proposed. The type strain is P15 ( = MTCC 8596 = CCM 7430).

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2011-09-01
2019-12-11
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vol. , part 9, pp. 2107 - 2111

Cellular fatty acid profiles of sp. nov. P15 and members of the genus .

Two-dimensional thin-layer chromatography of polar lipids of sp. nov. P15 .

Transmission electron micrograph of negatively stained cells of sp. nov. P15 showing a single polar flagellum.



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