1887

Abstract

A novel actinomycete, designated strain R-Ac121, was isolated from a small stone collected from an agricultural field in Jeju, Republic of Korea. The organism formed abundant vegetative mycelium that was branched and twisted. The reverse colour of colonies was brownish-yellow. Non-motile, wrinkled arthrospores were produced directly on the substrate mycelium. Aerial mycelium and sporangia were not observed. A neighbour-joining tree based on 16S rRNA gene sequences indicated that the isolate formed a distinct clade within the radiation of the family . The highest 16S rRNA gene sequence identity was found with the type strain of (97.3 % sequence similarity) followed by those of (97.0 %), (96.5 %), (96.5 %) and (96.4 %). The cell wall contained -diaminopimelic acid. The whole-cell sugars were rhamnose, glucose, ribose, xylose and arabinose. The polar lipids included diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The major menaquinone was MK-9(H) and the predominant fatty acids were iso-C, C, C 8 and 10-methyl C. The DNA G+C content was 71.8 mol%. The combination of morphological, chemotaxonomic and phylogenetic data clearly supports the separation of the organism from recognized species of the genus and related genera. On the basis of the data presented here, strain R-Ac121 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain R-Ac121 (=KCTC 19488 =DSM 45225).

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2010-11-01
2019-10-20
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