1887

Abstract

A novel thermophilic, anaerobic and organotrophic bacterium, designated strain MC3, was isolated from a coastal thermal spring on Île Saint-Paul in the Southern Indian Ocean. Cells of strain MC3 were motile rods, 0.8–1.0 μm wide and 1.0–2.4 μm long during exponential phase and up to 7.0 μm long during stationary phase. Strain MC3 was an anaerobic organotroph able to use diverse organic compounds. It was also able to reduce sulfur to sulfide. Growth was observed at temperatures ranging from 45 to 70 °C (optimum at 60 °C), between pH 5.5 and 7.5 (optimum at pH 6) and from 8 to 46 g NaCl l (optimum at 26 g l). The total G+C content of the genomic DNA was 26.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain MC3 was affiliated with the genus within the order . It shared 94.4–95.7 % 16S rRNA gene sequence similarity with strains of other species; was found to be the most closely related organism. Based on the data from the phylogenetic analysis and the physiological properties of the novel isolate, strain MC3 should be classified as a representative of a novel species, for which the name sp. nov. is proposed; the type strain is MC3 (=DSM 21709 =JCM 15581).

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2010-08-01
2020-09-23
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