%0 Journal Article %A Kaur, Jaspreet %A Verma, Mansi %A Lal, Rup %T Rhizobium rosettiformans sp. nov., isolated from a hexachlorocyclohexane dump site, and reclassification of Blastobacter aggregatus Hirsch and Müller 1986 as Rhizobium aggregatum comb. nov. %D 2011 %J International Journal of Systematic and Evolutionary Microbiology, %V 61 %N 5 %P 1218-1225 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.017491-0 %I Microbiology Society, %X A Gram-negative, rod-shaped, motile, aerobic bacterial strain, W3T, was isolated from hexachlorocyclohexane (HCH)-contaminated groundwater from Lucknow, India, and its taxonomic position was determined using a polyphasic approach. Strain W3T shared highest 16S rRNA gene sequence similarity of 97.8 % with Rhizobium selenitireducens B1T, followed by Rhizobium daejeonense L61T (97.7 %), Rhizobium radiobacter ATCC 19358T (97.5 %) and Blastobacter aggregatus IFAM 1003T (97.2 %). Strain W3T formed a monophyletic clade with Blastobacter aggregatus IFAM 1003T ( = DSM 1111T) in the cluster of species of the genus Rhizobium. Phylogenetic analyses of strain W3T using atpD and recA gene sequences confirmed the phylogenetic arrangements obtained by using 16S rRNA gene sequences. Hence, the taxonomic characterization of B. aggregatus DSM 1111T was also undertaken. Strains W3T and B. aggregatus DSM 1111T contained summed feature 8 (18 : 1ω7c and/or 18 : 1ω6c; 65.4 and 70.8 %, respectively) as the major fatty acid, characteristic of the genus Rhizobium. DNA–DNA relatedness of strain W3T with Rhizobium selenitireducens LMG 24075T, Rhizobium daejeonense DSM 17795T, Rhizobium radiobacter DSM 30147T and B. aggregatus DSM 1111T was 42, 34, 30 and 34 %, respectively. The DNA G+C contents of strain W3T and B. aggregatus DSM 1111T were 62.3 and 62.7 mol%, respectively. A nifH gene encoding dinitrogenase reductase was detected in strain W3T but not in B. aggregatus DSM 1111T. Based on the results obtained by phylogenetic and chemotaxonomic analyses, phenotypic characterization and DNA–DNA hybridization, it is concluded that strain W3T represents a novel species of the genus Rhizobium, for which the name Rhizobium rosettiformans sp. nov. is proposed (type strain W3T  = CCM 7583T  = MTCC 9454T). It is also proposed that Blastobacter aggregatus Hirsch and Müller 1986 be transferred to the genus Rhizobium as Rhizobium aggregatum comb. nov. (type strain IFAM 1003T  = DSM 1111T  = ATCC 43293T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.017491-0