1887

Abstract

A Gram-negative, rod-shaped, motile, aerobic bacterial strain, W3, was isolated from hexachlorocyclohexane (HCH)-contaminated groundwater from Lucknow, India, and its taxonomic position was determined using a polyphasic approach. Strain W3 shared highest 16S rRNA gene sequence similarity of 97.8 % with B1, followed by L61 (97.7 %), ATCC 19358 (97.5 %) and IFAM 1003 (97.2 %). Strain W3 formed a monophyletic clade with IFAM 1003 ( = DSM 1111) in the cluster of species of the genus . Phylogenetic analyses of strain W3 using and gene sequences confirmed the phylogenetic arrangements obtained by using 16S rRNA gene sequences. Hence, the taxonomic characterization of DSM 1111 was also undertaken. Strains W3 and DSM 1111 contained summed feature 8 (18 : 1ω7 and/or 18 : 1ω6; 65.4 and 70.8 %, respectively) as the major fatty acid, characteristic of the genus . DNA–DNA relatedness of strain W3 with LMG 24075, DSM 17795, DSM 30147 and DSM 1111 was 42, 34, 30 and 34 %, respectively. The DNA G+C contents of strain W3 and DSM 1111 were 62.3 and 62.7 mol%, respectively. A gene encoding dinitrogenase reductase was detected in strain W3 but not in DSM 1111. Based on the results obtained by phylogenetic and chemotaxonomic analyses, phenotypic characterization and DNA–DNA hybridization, it is concluded that strain W3 represents a novel species of the genus , for which the name sp. nov. is proposed (type strain W3  = CCM 7583  = MTCC 9454). It is also proposed that Hirsch and Müller 1986 be transferred to the genus as comb. nov. (type strain IFAM 1003  = DSM 1111  = ATCC 43293).

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2011-05-01
2019-10-16
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vol. , part 5, pp. 1218 - 1225

Fatty acid compositions of strain W3 , DSM 1111 and related type strains

Strains: 1, strain W3 ; 2, DSM 1111 ; 3, LMG 24075 ; 4, DSM 17795 ; 5, DSM 30147 . Data were obtained in this study. Values are percentages of total fatty acids. –, Not detected; tr, traces.

*Summed feature 2 comprises one or more of 12:0 aldehyde, iso-16:1 I and 14:0 3-OH. †Summed feature 3 comprises 16:1ω7 and/or 16:1ω6 . ‡Summed feature 8 comprises 18:1ω7 and/or 18:1ω6 .

(a–b) Transmission electron micrographs of cells of strain W3 (a) and DSM 1111 (b), after growth at 28 °C on LB agar for 48 h. Bars, 1 µm. (c) Photomicrograph of Gram-stained cells of strain W3 grown on LB medium at 28 °C for 48 h, showing rosette formation (arrow). Original magnification, ×1000. (d) Transmission electron micrograph of strain W3 grown on LB medium at 28 °C for 48 h, showing individual cells in rosette formation attached to each other by their poles. Bar, 1 µm.



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