1887

Abstract

Bacterial strains belonging to DNA hybridization groups (HG) II, IV and V, in the complex, of Brenner [ (1984), 45–55] were suggested previously to belong to the genus , but have never been formally described and classified. Additionally, it has been shown in several studies that is more closely related to species of than to those of . In this study, the phylogenetic positions of Brenner's DNA HG II, IV and V and were re-examined by both 16S rRNA gene sequencing and multilocus sequence analyses (MLSA) based on the , , and genes. The analyses revealed that DNA HG II, IV and V and form five separate branches within the genus (strains from HG V were split into two branches). DNA–DNA hybridization data further confirmed that DNA HG II, IV and V constitute four separate species. was shown to be a close phylogenetic relative of and DNA HG IV by both 16S rRNA gene sequence and MLSA analyses. Biochemical analyses performed on strains from DNA HG II, IV and V and confirmed their taxonomic position within the genus and revealed phenotypic characteristics that allow the differentiation of these species from each other and from their closest phylogenetic neighbours. It is proposed to emend the description of the genus and to describe sp. nov. for DNA HG II (type strain LMG 5345 =BD 874 =CDC 3123-70), sp. nov. for DNA HG IV (type strain LMG 2781 =BD 872 =CDC 1741-71 =LMG 5346), sp. nov. for strains of DNA HG V excluding LMG 24534 (type strain LMG 5343 =BD 873 =CDC 3482-71) and sp. nov. for the remaining strain of DNA HG V (type strain LMG 24534 =BD 805 =CDC 3527-71) and to transfer to the genus as comb. nov. (type strain LMG 2657 =ATCC 29267 =DSM 3873 =LMG 2655).

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2010-10-01
2019-12-15
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Supplements

vol. , part 10, pp. 2430 - 2440

GenBank accession numbers assigned to sequences obtained in this study.

UPGMA dendrogram constructed from 50 phenotypic characteristics useful for the differentiation of species.

[PDF file of Supplementary Table and Figure](71 KB)



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