1887

Abstract

A Gram-negative, aerobic bacterium, designated strain BZ26, was isolated from hydrocarbon-contaminated soil. The strain was psychrophilic, showing good growth over a temperature range of 1–20 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ26 was related to members of the genus and had highest 16S rRNA gene sequence similarity to 12116 (98.1 %), KCTC 12537 (97.5 %) and Gsoil 043 (97.2 %). Strain BZ26 had MK-7 as the major menaquinone and summed feature 3 (C 7/iso-C 2-OH), C 5 and iso-C as major fatty acids. The genomic DNA G+C content of strain BZ26 was 48.9 mol%. On the basis of phenotypic characteristics and genotypic analysis, strain BZ26 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BZ26 (=DSM 22270 =CGMCC 1.8951).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.017236-0
2010-07-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/7/1640.html?itemId=/content/journal/ijsem/10.1099/ijs.0.017236-0&mimeType=html&fmt=ahah

References

  1. Baik, K. S., Kim, M. S., Kim, E. M., Kim, H. R. & Seong, C. N. ( 2007; ). Dyadobacter koreensis sp. nov., isolated from fresh water. Int J Syst Evol Microbiol 57, 1227–1231.[CrossRef]
    [Google Scholar]
  2. Chaturvedi, P., Reddy, G. S. N. & Shivaji, S. ( 2005; ). Dyadobacter hamtensis sp. nov., from Hamta glacier, located in the Himalayas, India. Int J Syst Evol Microbiol 55, 2113–2117.[CrossRef]
    [Google Scholar]
  3. Chelius, M. K. & Triplett, E. W. ( 2000; ). Dyadobacter fermentans gen. nov., sp. nov., a novel Gram-negative bacterium isolated from surface-sterilized Zea mays stems. Int J Syst Evol Microbiol 50, 751–758.[CrossRef]
    [Google Scholar]
  4. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  5. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  6. Dong, Z., Guo, X., Zhang, X., Qiu, F., Sun, L., Gong, H. & Zhang, F. ( 2007; ). Dyadobacter beijingensis sp. nov., isolated from the rhizosphere of turf grasses in China. Int J Syst Evol Microbiol 57, 862–865.[CrossRef]
    [Google Scholar]
  7. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  8. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  9. Liu, Q.-M., Im, W.-T., Lee, M., Yang, D.-C. & Lee, S.-T. ( 2006; ). Dyadobacter ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 56, 1939–1944.[CrossRef]
    [Google Scholar]
  10. Margesin, R., Zacke, G. & Schinner, F. ( 2002; ). Characterization of heterotrophic microorganisms in alpine glacier cryoconite. Arct Antarct Alp Res 34, 88–93.[CrossRef]
    [Google Scholar]
  11. Margesin, R., Gander, S., Zacke, G., Gounot, A. M. & Schinner, F. ( 2003; ). Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. Extremophiles 7, 451–458.[CrossRef]
    [Google Scholar]
  12. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  13. Reddy, G. S. N. & Garcia-Pichel, F. ( 2005; ). Dyadobacter crusticola sp. nov., from biological soil crusts in the Colorado Plateau, USA, and an emended description of the genus Dyadobacter Chelius and Triplett 2000. Int J Syst Evol Microbiol 55, 1295–1299.[CrossRef]
    [Google Scholar]
  14. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  15. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  16. Tang, Y., Dai, J., Zhang, L., Mo, Z., Wang, Y., Li, Y., Ji, S., Fang, C. & Zheng, C. ( 2009; ). Dyadobacter alkalitolerans sp. nov., isolated from desert sand. Int J Syst Evol Microbiol 59, 60–64.[CrossRef]
    [Google Scholar]
  17. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J. ( 1989; ). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178.
    [Google Scholar]
  18. Zhang, D.-C., Wang, H.-X., Liu, H.-C., Dong, X.-Z. & Zhou, P.-J. ( 2006; ). Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier. Int J Syst Evol Microbiol 56, 2921–2925.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.017236-0
Loading
/content/journal/ijsem/10.1099/ijs.0.017236-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error