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Abstract

The taxonomic position of a bright orange-pigmented bacterial strain, designated CC-GZM-130, isolated from a water sample of the Guan-zing-ling hot spring, southern Taiwan, was studied. The strain was able to grow on nutrient agar at 25–40 °C and in the presence of 1–3 % (w/v) NaCl. Comparative analyses of the 16S rRNA gene sequence showed that the isolate was grouped in the vicinity of the genus with the highest sequence similarity of 92.1 % to the type strain of , followed by sequence similarities of 92.0, 91.6 and 91.5 % to the type strains of , and , respectively. The polyamine pattern showed that the major compound was -homospermidine. The quinone system was menaquinone MK-7. The polar lipid profile was composed predominantly of phosphatidylethanolamine, three polar lipids and one aminolipid. Minor amounts of other lipids were also detectable. The main characteristics of the fatty acid profiles of strain CC-GZM-130, and were similar, with iso-C, iso-C 9 and iso-C 3-OH as the major fatty acids, but some qualitative and quantitative differences were observed. The DNA GC content of the novel strain was 53.2 mol%. The isolate clearly differed genotypically and phenotypically from representatives of the most closely related genera. On the basis of these differences, a novel species in a new genus, gen. nov., sp. nov., is proposed with CC-GZM-130 (=CCUG 57694=CCM 7650) as the type strain of the type species.

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2010-09-01
2019-12-11
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References

  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J. ( 1996; ). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef]
    [Google Scholar]
  2. Brettar, I., Christen, R. & Höfle, M. G. ( 2004a; ). Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the Cytophaga-Flavobacterium-Bacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54, 65–70.[CrossRef]
    [Google Scholar]
  3. Brettar, I., Christen, R. & Höfle, M. G. ( 2004b; ). Aquiflexum balticum gen. nov., sp. nov., a novel marine bacterium of the Cytophaga-Flavobacterium-Bacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54, 2335–2341.[CrossRef]
    [Google Scholar]
  4. Busse, H.-J. & Auling, G. ( 1988; ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef]
    [Google Scholar]
  5. Chung, Y. C., Kobayashi, T., Kanai, H., Akiba, T. & Kudo, T. ( 1995; ). Purification and properties of extracellular amylase from the hyperthermophilic archeon Thermococcus profundus DT5432. Appl Environ Microbiol 61, 1502–1506.
    [Google Scholar]
  6. Collins, M. D. ( 1985; ). Analysis of isoprenoid quinones. Methods Microbiol 18, 329–366.
    [Google Scholar]
  7. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  8. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  9. Kämpfer, P., Steiof, M. & Dott, W. ( 1991; ). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P., Dreyer, U., Neef, A., Dott, W. & Busse, H.-J. ( 2003; ). Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53, 93–97.[CrossRef]
    [Google Scholar]
  11. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  12. Ludwig, W., Euzéby, J. & Whitman, W. B. ( 2008; ). Draft taxonomic outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi and Gemmatimonadetes for Volume 4 of the second edition of Bergey's Manual of Systematic Bacteriology. http://www.bergeys.org/outlines/Bergeys_Vol_4_Outline.pdf.
  13. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  14. Olsen, G. J., Matsuda, H., Hagstrom, R. & Overbeek, R. ( 1994; ). fastDNAml: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci 10, 41–48.
    [Google Scholar]
  15. Pruesse, E., Quast, C., Knittel, K., Fuchs, B. M., Ludwig, W., Peplies, J. & Glöckner, F. O. ( 2007; ). SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res 35, 7188–7196.[CrossRef]
    [Google Scholar]
  16. Stolz, A., Busse, H.-J. & Kämpfer, P. ( 2007; ). Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57, 572–576.[CrossRef]
    [Google Scholar]
  17. Strunk, O., Gross, O., Reichel, B. & other authors ( 2000; ). arb: a software environment for sequence data. Department of Microbiology, Technische Universität München, Munich, Germany. http://www.arb-home.de.
  18. Tindall, B. J. ( 1990a; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef]
    [Google Scholar]
  19. Tindall, B. J. ( 1990b; ). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef]
    [Google Scholar]
  20. Young, C.-C., Lin, S.-Y., Arun, A. B., Shen, F.-T., Chen, W.-M., Rekha, P. D., Langer, S., Busse, H.-J. & Kämpfer, P. ( 2009; ). Algoriphagus olei sp. nov., isolated from oil-contaminated soil. Int J Syst Evol Microbiol 59, 2909–2915.[CrossRef]
    [Google Scholar]
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Neighbour-joining tree based on 16S rRNA gene sequences showing the relationship of strain CC-GZM-130 and representative members of the family ‘ ’. [ PDF] 44 KB

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The chemical composition of water samples from Guan-zing-ling hot spring, Taiwan, from where the type strain was isolated. Phenotypic traits distinguishing strain CC-GZM-130 from related taxa. [ Combined PDF] 45 KB

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