A Gram-negative, aerobic, yellow-pigmented, rod-shaped, non-motile bacterium, strain KMM 3895T, was isolated from a marine sandy sample collected offshore from the Sea of Japan. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain KMM 3895T in the class Gammaproteobacteria, forming a separate branch, sharing 89.5 % sequence similarity with Nitrincola lacisaponensis 4CAT and 88–87 % similarity with the other members of the cluster, including members of Kangiella, Spongiibacter, Alcanivorax and Microbulbifer. The major isoprenoid quinone was Q-8. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminophospholipid and an unknown phospholipid. Fatty acid analysis revealed C16 : 1ω7, iso-C16 : 0, iso-C18 : 0 and C18 : 1ω7 as dominant components. The DNA G+C content was determined to be 48.1 mol%. Based on its unique phenotypic characteristics and phylogenetic distance, the marine coastal sediment isolate KMM 3895T should be classified as a representative of a novel genus and species, for which the name Arenicella xantha gen. nov., sp. nov. is proposed. The type strain of Arenicella xantha is KMM 3895T (=NRIC 0759T =JCM 16153T).
DimitriuP. A.,
ShuklaS. K.,
ConradtJ.,
MárquezM. C.,
VentosaA.,
MagliaA.,
PeytonB. M.,
PinkartH. C.,
MormileM. R.2005; Nitrincola lacisaponensis gen. nov., sp. nov., a novel alkaliphilic bacterium isolated from an alkaline, saline lake. Int J Syst Evol Microbiol 55:2273–2278[CrossRef]
Fernández-MartínezJ.,
PujalteM. J.,
García-MartínezJ.,
MataM.,
GarayE.,
Rodríguez-ValeraF.2003; Description of Alcanivorax venustensis sp. nov. and reclassification of Fundibacter jadensis DSM 12178T (Bruns and Berthe-Corti 1999) as Alcanivorax jadensis comb. nov., members of the emended genus Alcanivorax
. Int J Syst Evol Microbiol 53:331–338[CrossRef]
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
(editors) 1994Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
GonzálezJ. M.,
MayerF.,
MoranA.,
HodsonR. E.,
WhitmanW. B.1997; Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47:369–376[CrossRef]
MarmurJ.,
DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118[CrossRef]
RomanenkoL. A.,
UchinoM.,
FalsenE.,
FrolovaG. M.,
ZhukovaN. V.,
MikhailovV. V.2005; Pseudomonas pachastrellae sp. nov. isolated from a marine sponge. Int J Syst Evol Microbiol 55:919–924[CrossRef]
ShidaO.,
TakagiH.,
KadowakiK.,
NakamuraL. K.,
KomagataK.1997; Transfer of Bacillus alginolyticus , Bacillus chondroitinus , Bacillus curdlanolyticus , Bacillus glucanolyticus , Bacillus kobensis , and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus
. Int J Syst Bacteriol 47:289–298[CrossRef]
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology
. pp 607–654 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
TamuraK.,
DudleyJ.,
NeiM.,
KumarS.2007; mega 4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599[CrossRef]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
YakimovM. M.,
GolyshinP. N.,
LangS.,
MooreE. R. B.,
AbrahamW.-R.,
LünsdorfH.,
TimmisK. N.1998; Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. Int J Syst Bacteriol 48:339–348[CrossRef]
YoonJ.-H.,
KimI.-G.,
OhT.-K.,
ParkY.-H.2004a; Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea. Int J Syst Evol Microbiol 54:1111–1116[CrossRef]
YoonJ.-H.,
OhT.-K.,
ParkY.-H.2004b; Kangiella koreensis gen. nov., sp. nov. and Kangiella aquimarina sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54:1829–1835[CrossRef]