1887

Abstract

A Gram-negative, aerobic, yellow-pigmented, rod-shaped, non-motile bacterium, strain KMM 3895, was isolated from a marine sandy sample collected offshore from the Sea of Japan. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain KMM 3895 in the class , forming a separate branch, sharing 89.5 % sequence similarity with 4CA and 88–87 % similarity with the other members of the cluster, including members of , , and . The major isoprenoid quinone was Q-8. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminophospholipid and an unknown phospholipid. Fatty acid analysis revealed C 7, iso-C, iso-C and C 7 as dominant components. The DNA G+C content was determined to be 48.1 mol%. Based on its unique phenotypic characteristics and phylogenetic distance, the marine coastal sediment isolate KMM 3895 should be classified as a representative of a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is KMM 3895 (=NRIC 0759 =JCM 16153).

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2010-08-01
2019-12-08
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Two-dimensional TLC of polar lipids of strain KMM 3895 . DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; APL, aminophospholipid; PL, unknown phopholipid.

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Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences showing relationships of isolate KMM 3895 and related members of the . [PDF](43 KB)

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