RT Journal Article SR Electronic(1) A1 Sravan Kumar, R. A1 Sasi Jyothsna, T. S. A1 Sasikala, Ch. A1 Seong, C. N. A1 Lim, C. H. A1 Park, S. C. A1 Ramana, Ch. V.YR 2010 T1 Shewanella fodinae sp. nov., isolated from a coal mine and from a marine lagoon JF International Journal of Systematic and Evolutionary Microbiology, VO 60 IS 7 SP 1649 OP 1654 DO https://doi.org/10.1099/ijs.0.017046-0 PB Microbiology Society, SN 1466-5034, AB Strains JC15T and JC19 were isolated from samples collected from different locations in India, including a coal mine and a marine lagoon. Both strains were Gram-stain-negative rods, motile by means of a single polar flagellum, catalase- and oxidase-positive, and hydrolysed casein, produced H2S and showed β-haemolysis. Strain JC15T grew optimally at pH 6 (range pH 5–8) while strain JC19 grew optimally at pH 7 (range pH 6-9) and both had a growth temperature optimum of 30–37 °C (range 22–40 °C). Both strains could grow chemo-organoheterotrophically and chemolithoautotrophically. Neither strain required NaCl for growth and both could tolerate up to 9 % (w/v) NaCl, with optimum growth at 5 % NaCl. Vitamin B12 was required as a growth factor by both strains. The major fatty acids were iso-C15 : 0, C17 : 1 ω8c and iso-C13 : 0 3-OH. The DNA G+C contents of strains JC15T and JC19 were 53.6 and 54.3 mol%, respectively. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strains JC15T and JC19 were most closely related to Shewanella haliotis DW01T (approximately 94 % sequence similarity) and to other members of the genus Shewanella. Genomic relatedness (DNA–DNA hybridization) between strains JC15T and JC19 is 88 %. On the basis of phenotypic and molecular genetic evidence, strain JC15T represents a novel species of the genus Shewanella, for which the name Shewanella fodinae sp. nov. is proposed. The type strain is JC15T (=CCUG 57102T =NBRC 105216T =KCTC 22506T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.017046-0