%0 Journal Article %A Sravan Kumar, R. %A Sasi Jyothsna, T. S. %A Sasikala, Ch. %A Seong, C. N. %A Lim, C. H. %A Park, S. C. %A Ramana, Ch. V. %T Shewanella fodinae sp. nov., isolated from a coal mine and from a marine lagoon %D 2010 %J International Journal of Systematic and Evolutionary Microbiology, %V 60 %N 7 %P 1649-1654 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.017046-0 %I Microbiology Society, %X Strains JC15T and JC19 were isolated from samples collected from different locations in India, including a coal mine and a marine lagoon. Both strains were Gram-stain-negative rods, motile by means of a single polar flagellum, catalase- and oxidase-positive, and hydrolysed casein, produced H2S and showed β-haemolysis. Strain JC15T grew optimally at pH 6 (range pH 5–8) while strain JC19 grew optimally at pH 7 (range pH 6-9) and both had a growth temperature optimum of 30–37 °C (range 22–40 °C). Both strains could grow chemo-organoheterotrophically and chemolithoautotrophically. Neither strain required NaCl for growth and both could tolerate up to 9 % (w/v) NaCl, with optimum growth at 5 % NaCl. Vitamin B12 was required as a growth factor by both strains. The major fatty acids were iso-C15 : 0, C17 : 1 ω8c and iso-C13 : 0 3-OH. The DNA G+C contents of strains JC15T and JC19 were 53.6 and 54.3 mol%, respectively. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strains JC15T and JC19 were most closely related to Shewanella haliotis DW01T (approximately 94 % sequence similarity) and to other members of the genus Shewanella. Genomic relatedness (DNA–DNA hybridization) between strains JC15T and JC19 is 88 %. On the basis of phenotypic and molecular genetic evidence, strain JC15T represents a novel species of the genus Shewanella, for which the name Shewanella fodinae sp. nov. is proposed. The type strain is JC15T (=CCUG 57102T =NBRC 105216T =KCTC 22506T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.017046-0