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Abstract

Strains JC15 and JC19 were isolated from samples collected from different locations in India, including a coal mine and a marine lagoon. Both strains were Gram-stain-negative rods, motile by means of a single polar flagellum, catalase- and oxidase-positive, and hydrolysed casein, produced HS and showed -haemolysis. Strain JC15 grew optimally at pH 6 (range pH 5–8) while strain JC19 grew optimally at pH 7 (range pH 6-9) and both had a growth temperature optimum of 30–37 °C (range 22–40 °C). Both strains could grow chemo-organoheterotrophically and chemolithoautotrophically. Neither strain required NaCl for growth and both could tolerate up to 9 % (w/v) NaCl, with optimum growth at 5 % NaCl. Vitamin B was required as a growth factor by both strains. The major fatty acids were iso-C, C 8 and iso-C 3-OH. The DNA GC contents of strains JC15 and JC19 were 53.6 and 54.3 mol%, respectively. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strains JC15 and JC19 were most closely related to DW01 (approximately 94 % sequence similarity) and to other members of the genus Genomic relatedness (DNA–DNA hybridization) between strains JC15 and JC19 is 88 %. On the basis of phenotypic and molecular genetic evidence, strain JC15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC15 (=CCUG 57102 =NBRC 105216 =KCTC 22506).

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2010-07-01
2019-10-21
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TEM photograph of a cell of strain JC15 showing the single polar flagellum.

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NaCl optima and tolerance of strains JC15 and JC19.

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. Results of GN2 microplate (Biolog) test for strain JC15 after 24h of incubation at 30 °C. [ PDF] (89 KB)

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