The omission of the name ‘Mycobacterium paraffinicum’ from the Approved Lists of Bacterial Names was due to phenotypic confusion surrounding a close relationship with Mycobacterium scrofulaceum. Correspondingly, ‘M. paraffinicum’ strains grew slowly in >7 days, stained acid–alcohol-fast and produced yellow-pigmented, smooth, waxy colonies in the dark at an optimal temperature of 35 °C. However, ‘M. paraffinicum’ strains demonstrated no activity for urease, nicotinamidase or pyrazinamidase and lacked growth at 42 °C, unlike M. scrofulaceum. The mycolic acid pattern, as determined by HPLC, clustered ‘M. paraffinicum’ with M. scrofulaceum, Mycobacterium avium and Mycobacterium parascrofulaceum. Strains were fully susceptible to linezolid, rifabutin, clarithromycin and amikacin. Examination of the historical reference strain of ‘M. paraffinicum’, ATCC 12670, and five additional isolates using comparative studies with 16S rRNA, hsp65 and rpoB gene and concatenated sequences showed that they formed a tight taxonomic group that was distinct from similar non-tuberculous mycobacteria. Multilocus enzyme electrophoresis (MEE) analysis confirmed a close association of the five additional isolates with the reference strain of ‘M. paraffinicum’ with a genetic distance of 0.12 and showed that all six strains were distinct from other closely related species. These genetic results provided unambiguous evidence of the uniqueness of this slowly growing, scotochromogenic species and supported the revival of the name as Mycobacterium paraffinicum (ex Davis, Chase and Raymond 1956) sp. nov., nom. rev. We propose the previously deposited reference strain ATCC 12670T =DSM 44181T =NCIMB 10420T, located in collections worldwide, as the type strain.
AdékambiT.,
DrancourtM.2004; Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65 , recA and rpoB gene sequencing. Int J Syst Evol Microbiol 54:2095–2105[CrossRef]
ButlerW. R.,
GuthertzL. S.2001; Mycolic acid analysis by high-performance liquid chromatography for identification of Mycobacterium species. Clin Microbiol Rev 14:704–726[CrossRef]
CharlesN.,
ToneyS. R.,
ButlerW. R.2005Identification of mycobacteria by 16S rRNA gene sequencing and HPLC analysis of mycolic acids in a reference laboratory . In Abstracts of the 105th General Meeting of the American Society for Microbiology , 5–10 June 2005, Atlanta, GA, USA, abstract U-042 Washington, DC: American Society for Microbiology;
GusevM. V.,
KoronelliT. V.,
KorolevI. N.1978; Study of the dynamics of the uptake and utilization of a hydrocarbon in Mycobacterium paraffinicum cells by means of IR-spectroscopy. Mikrobiologiia 47:1025–1029 (in Russian)
HafnerB. H.,
HaagH.,
GeissH-K.,
NolteO.2004; Different molecular methods for the identification of rarely isolated non-tuberculous mycobacteria and description of new hsp65 restriction fragment length polymorphism patterns. Mol Cell Probes 18:59–65[CrossRef]
HeifetsL.1988; MIC as a quantitative measurement of the susceptibility of Mycobacterium avium strains to seven antituberculosis drugs. Antimicrob Agents Chemother 32:1131–1136[CrossRef]
KentP. T.,
KubicaG. P.1985Public Health Mycobacteriology: a Guide for the Level III Laboratory Atlanta: Centers for Disease Control, US Department of Public Health & Human Services;
LapageS. P.,
SneathP. H. A.,
LesselE. F.,
SkermanV. B. D.,
SeeligerH. P. R.,
ClarkW. A.
(editors) 1992International Code of Nomenclature of Bacteria (1990 revision). Bacteriological Code Washington, DC: American Society for Microbiology;
LefmannM.,
HonischC.,
BöckerS.,
StormN.,
von WintzingerodeF.,
SchlötelburgC.,
MoterA.,
van den BoomD.,
GöbelU. B.2004; Novel mass spectrometry-based tool for genotypic identification of mycobacteria. J Clin Microbiol 42:339–346[CrossRef]
Lévy-FrébaultV. V.,
PortalsF.1998; Proposed minimal standards for the genus Mycobacterium and for description of new slowly growing Mycobacterium species. Int J Syst Bacteriol 42:315–323
McNabbA.,
EislerD.,
AdieK.,
AmosM.,
RodriguesM.,
StephensG.,
BlackW. A.,
Isaac-RentonJ.2004; Assessment of partial sequencing of the 65-kilodalton heat shock protein gene (hsp65) for routine identification of Mycobacterium species isolated from clinical sources. J Clin Microbiol 42:3000–3011[CrossRef]
NCCLS2003Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes. Approved Standard. NCCLS document M24-A. (ISBN 1-56238-500-3) Wayne, PA: National Committee on Clinical Laboratory Standards;
RogallT.,
WoltersJ.,
FlohrT.,
BöttgerE. C.1990; Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium
. Int J Syst Bacteriol 40:323–330[CrossRef]
RussoC.,
TortoliE.,
MenichellaD.2006; Evaluation of the new GenoType Mycobacterium assay for identification of mycobacterial species. J Clin Microbiol 44:334–339[CrossRef]
SehrtI.,
KapplerW.,
LangeA.1976; Differentiation and identification of mycobacteria by means of thin-layer chromatography of their lipids. III. Application for the classification of scotochromogenic mycobacteria. Z Erkr Atmungsorgane 145:196–201 (in German
SpringerB.,
TortoliE.,
RichterI.,
GrünewaldR.,
Rüsch-GerdesS.,
UschmannK.,
SuterF.,
CollinsM. D.,
KroppenstedtR. M.,
BöttgerE. C.1995; Mycobacterium conspicuum sp. nov., a new species isolated from patients with disseminated infections. J Clin Microbiol 33:2805–2811
TelentiA.,
MarchesiF.,
BalzM.,
BallyF.,
BöttgerE.,
BodmerT.1993; Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31:175–178
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
WallaceR. J.Jr,
NashD. R.,
SteeleL. C.,
SteingrubeV.1986; Susceptibility testing of slowly growing mycobacteria by microdilution MIC method with 7H9 broth. J Clin Microbiol 24:976–981
WangS.-H.,
ManginoJ. E.,
StevensonK.,
YakrusM. A.,
CookseyR.,
ButlerW. R.,
HealyM.,
WiseM. G.,
SchlesingerL. S.,
PancholiP.2008; Characterization of a “ Mycobacterium paraffinicum ” isolate associated with a pseudo-outbreak. J Clin Microbiol 46:1850–1853[CrossRef]
WayneL. G.,
DietzT. M.,
Gernez-RieuxC.,
JenkinsP. A.,
KäpplerW.,
KubicaG. P.,
KwapinskiJ. B. G.,
MeissnerG.,
PattynS. R.other authors1971; A cooperative numerical analysis of scotochromogenic slowly growing mycobacteria. J Gen Microbiol 66:255–271[CrossRef]
WayneL. G.,
GoodR. C.,
KrichevskyM. I.,
BeamR. E.,
BlacklockZ.,
ChaparasS. D.,
DawsonD.,
FormanS. D.,
GrossW.other authors1981; First report of the cooperative, open-ended study of slowly growing mycobacteria by the International Working Group on Mycobacterial Taxonomy. Int J Syst Bacteriol 31:1–20[CrossRef]
WayneL. G.,
GoodR. C.,
KrichevskyM. I.,
BeamR. E.,
BlacklockZ.,
DavidH. L.,
DawsonD.,
GrossW.,
HawkinsJ.other authors1983; Second report of the cooperative, open-ended study of slowly growing mycobacteria by the International Working Group on Mycobacterial Taxonomy. Int J Syst Bacteriol 33:265–274[CrossRef]
WayneL. G.,
GoodR. C.,
KrichevskyM. I.,
BlacklockZ.,
DavidH. L.,
DawsonD.,
GrossW.,
HawkinsJ.,
JenkinsP. A.other authors1989; Third report of the cooperative, open-ended study of slowly growing mycobacteria by the International Working Group on Mycobacterial Taxonomy. Int J Syst Bacteriol 39:267–278[CrossRef]
WayneL. G.,
GoodR. C.,
KrichevskyM. I.,
BlacklockZ.,
DavidH. L.,
DawsonD.,
GrossW.,
HawkinsJ.,
Levy-FrebaultV. V.other authors1991; Fourth report of the cooperative, open-ended study of slowly growing mycobacteria by the International Working Group on Mycobacterial Taxonomy. Int J Syst Bacteriol 41:463–472[CrossRef]
WayneL. G.,
GoodR. C.,
BöttgerE. C.,
ButlerR.,
DorschM.,
EzakiT.,
GrossW.,
JonasV.,
KilburnJ.other authors1996; Semantide- and chemotaxonomy-based analyses of some problematic phenotypic clusters of slowly growing mycobacteria, a cooperative study of the International Working Group on Mycobacterial Taxonomy. Int J Syst Bacteriol 46:280–297[CrossRef]
YakrusM. A.,
ReevesM. W.,
HunterS. B.1992; Characterization of isolates of Mycobacterium avium serotypes 4 and 8 from patients with AIDS by multilocus enzyme electrophoresis. J Clin Microbiol 30:1474–1478
YoungJ. M.,
EuzébyJ. P.2008; Proposed revision of Rule 33c to perpetuate the citation of revived names. Int J Syst Evol Microbiol 58:2468–2469[CrossRef]