An actinomycete strain, designated YU1183-22T, was isolated from a compost sample collected in Nikko, Japan. The isolate formed white aerial mycelium with relatively long aerial hyphae showing chains of arthrospores. Strain YU1183-22T grew with 0–10 % (w/v) NaCl, at pH 6–11 and at 10–37 °C (optimum 30 °C). Strain YU1183-22T contained meso-diaminopimelic acid and no diagnostic sugars. The predominant menaquinones were MK-10(H10) and MK-10(H8). The polar lipids were phosphatidylcholine and phosphatidylglycerol. The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0 and tuberculostearic acid. The G+C content of the DNA was 72.3 mol%. Chemotaxonomic and morphological characterization clearly demonstrated that strain YU1183-22T belonged to the genus Nocardiopsis. Phylogenetic analysis using 16S rRNA gene sequences showed that the isolate was closely related to Nocardiopsis salina YIM 90010T (98.0 % 16S rRNA gene sequence similarity), Nocardiopsis xinjiangensis YIM 90004T (97.9 %) and Nocardiopsis kunsanensis HA-9T (97.3 %). However, DNA–DNA relatedness as well as physiological and biochemical analyses showed that strain YU1183-22T could be differentiated from its closest phylogenetic relatives. It is proposed that this strain be classified as a representative of a novel species of the genus Nocardiopsis, with the name Nocardiopsis nikkonensis sp. nov. The type strain is YU1183-22T (=NBRC 102170T =KCTC 19666T).
ChunJ.,
LeeJ.-H.,
JungY.,
KimM.,
KimS.,
KimB. K.,
LimY.-W.2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261[CrossRef]
GuindonS.,
LethiecF.,
DurouxP.,
GascuelO.2005; phyml online – a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Res 33 (Web Server Issue):W557–W559[CrossRef]
HatanoK.,
NishiiT.,
KasaiH.2003; Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium ) species by using phenotype, DNA–DNA hybridization and sequences of gyrB , and proposal of Streptomyces luteireticuli ( ex Katoh and Arai 1957) corrig. sp. nov., nom. rev.. Int J Syst Evol Microbiol531519–1529[CrossRef]
HayakawaM.,
NonomuraH.1987; Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65:501–509[CrossRef]
HayakawaM.,
IinoS.,
NonomuraH.1982; Heavy metal resistance and melanoid pigment production in the streptomycete flora of copper-polluted vineyard soils. Hakkokogaku Kaishi 60:1–9
KämpferP.,
BusseH. J.,
RaineyF. A.2002; Nocardiopsis compostus sp. nov., from the atmosphere of a composting facility. Int J Syst Evol Microbiol 52:621–627
KimJ. W.,
AdachiH.,
Shin-yaK.,
HayakawaY.,
SetoH.1997; Apoptolidin, a new apoptosis inducer in transformed cells from Nocardiopsis sp. J Antibiot (Tokyo 50:628–630[CrossRef]
LechevalierM. P.,
LechevalierH. A.1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443[CrossRef]
MinnikinD. E.,
O'DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241[CrossRef]
SchaalK. P.1985; Identification of clinically significant actinomycetes and related bacteria using chemical techniques. In Chemical Methods in Bacterial Systematics pp 359–381 Edited by
GoodfellowM.,
MinnikinD. E.
London: Academic Press;
SierraG.1957; A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates. Antonie van Leeuwenhoek 23:15–22[CrossRef]
TakahashiK.,
NeiM.2000; Efficiencies of fast algorithms of phylogenetic inference under the criteria of maximum parsimony, minimum evolution, and maximum likelihood when a large number of sequences are used. Mol Biol Evol 17:1251–1258[CrossRef]
TamuraT.,
HatanoK.2001; Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘ Actinoplanes aurantiacus ’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov.. Int J Syst Evol Microbiol 51:2119–2125[CrossRef]
TamuraT.,
NakagaitoY.,
NishiiT.,
HasegawaT.,
StackebrandtE.,
YokotaA.1994; A new genus of the order Actinomycetales , Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov.. Int J Syst Bacteriol 44:193–203[CrossRef]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.other authors1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]
WilliamsS. T.,
GoodfellowM.,
AldersonG.,
WellingtonE. M. H.,
SneathP. H. A.,
SackinM. J.1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
YassinA. F.,
RaineyF. A.,
BurghardtJ.,
GierthD.,
UngerechtsJ.,
LuxI.,
SeifertP.,
BalC.,
SchaalK. P.1997; Description of Nocardiopsis synnemataformans sp. nov., elevation of Nocardiopsis alba subsp. prasina to Nocardiopsis prasina comb. nov., and designation of Nocardiopsis antarctica and Nocardiopsis alborubida as later subjective synonyms of Nocardiopsis dassonvillei
. Int J Syst Bacteriol 47:983–988[CrossRef]