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Abstract

An actinomycete strain, designated YU1183-22, was isolated from a compost sample collected in Nikko, Japan. The isolate formed white aerial mycelium with relatively long aerial hyphae showing chains of arthrospores. Strain YU1183-22 grew with 0–10 % (w/v) NaCl, at pH 6–11 and at 10–37 °C (optimum 30 °C). Strain YU1183-22 contained -diaminopimelic acid and no diagnostic sugars. The predominant menaquinones were MK-10(H) and MK-10(H). The polar lipids were phosphatidylcholine and phosphatidylglycerol. The major cellular fatty acids were iso-C, anteiso-C and tuberculostearic acid. The G+C content of the DNA was 72.3 mol%. Chemotaxonomic and morphological characterization clearly demonstrated that strain YU1183-22 belonged to the genus . Phylogenetic analysis using 16S rRNA gene sequences showed that the isolate was closely related to YIM 90010 (98.0 % 16S rRNA gene sequence similarity), YIM 90004 (97.9 %) and HA-9 (97.3 %). However, DNA–DNA relatedness as well as physiological and biochemical analyses showed that strain YU1183-22 could be differentiated from its closest phylogenetic relatives. It is proposed that this strain be classified as a representative of a novel species of the genus , with the name sp. nov. The type strain is YU1183-22 (=NBRC 102170 =KCTC 19666).

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2010-12-01
2020-01-21
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vol. , part 12, pp. 2967 - 2971

Scanning electron micrograph of strain YU1183-22 grown on HV agar at 28°C for 14 days. Bar, 2 µm.



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