A Gram-negative, motile, non-spore-forming and lipolytic bacterial strain, designated Gung47T, was isolated from a tidal flat on the west coast of Korea. Strain Gung47T grew optimally at 30 °C and with 2−5 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain Gung47T belonged to the genus Photobacterium. Strain Gung47T exhibited 98.1 % 16S rRNA gene sequence similarity with Photobacterium rosenbergii LMG 22223T and 94.3–96.3 % similarity with other type strains of species of the genus Photobacterium. Strain Gung47T exhibited 47 % DNA–DNA relatedness to P. rosenbergii LMG 22223T. Strain Gung47T contained Q-8 as the predominant ubiquinone and C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c as the major fatty acids. In this study, two closely related type strains, P. rosenbergii LMG 22223T and Photobacteriumhalotolerans LMG 22194T, were also found to have Q-8 as the predominant ubiquinone. The DNA G+C content of strain Gung47T was 50.6 mol%. The differential phenotypic properties together with the phylogenetic and genetic distinctiveness of strain Gung47T demonstrated that this strain is distinguishable from recognized Photobacterium species. Therefore, strain Gung47T is considered to represent a novel species of the genus Photobacterium, for which the name Photobacterium gaetbulicola sp. nov. is proposed. The type strain is Gung47T (=KCTC 22804T =CCUG 58399T).
AnzaiY.,
KimH.,
ParkJ.-Y.,
WakabayashiH.,
OyaizuH.2000; Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589[CrossRef]
BaumannP.,
BaumannL.1981; The marine Gram-negative eubacteria: genera Photobacterium , Beneckea , Alteromonas , Pseudomonas , and Alcaligenes
. In The Prokaryotes pp 1302–1331 Edited by
StarrM. P.,
StolpH.,
TrüperH. G.,
BalowsA.,
SchlegelH. G.
Berlin: Springer;
BoisvertH.,
ChatelainR.,
BassotJ.-M.1967; Etude d'un Photobacterium isolé de l'organe lumineux de poissons Leiognathidae. Ann Inst Pasteur (Paris) 112:520–524 (in French)
BrunsA.,
RohdeM.,
Berthe-CortiL.2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol511997–2006[CrossRef]
ChunJ.,
LeeJ.-H.,
JungY.,
KimM.,
KimS.,
KimB. K.,
LimY.-W.2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261[CrossRef]
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229[CrossRef]
FarmerJ.III,
Hickman-BrennerF.2006; The genera Vibrio and Photobacterium
. In The Prokaryotes, 3rd edn. vol 6 pp 508–563 Edited by
DworkinM.,
FalkowS.,
RosenbergE.,
SchleiferK. H.,
StackebrandtE.
New York: Springer;
JungS.-Y.,
JungY.-T.,
OhT.-K.,
YoonJ.-H.2007; Photobacterium lutimaris sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 57:332–336[CrossRef]
NogiY.,
MasuiN.,
KatoC.1998; Photobacterium profundum sp. nov., a new, moderately barophilic bacterial species isolated from a deep-sea sediment. Extremophiles 2:1–7[CrossRef]
OnarheimA. M.,
WiikR.,
BurghardtJ.,
StackebrandtE.1994; Characterization and identification of two Vibrio species indigenous to the intestine of fish in cold sea water; description of Vibrio iliopiscarius sp. nov. Syst Appl Microbiol 17:370–379[CrossRef]
ReicheltJ. L.,
BaumannP.,
BaumannL.1976; Study of genetic relationships among marine species of the genera Beneckea and Photobacterium
. Arch Microbiol 110:101–120[CrossRef]
SmithS. K.,
SuttonD. C.,
FuerstJ. A.,
ReicheltJ. L.1991; Evaluation of the genus Listonella and reassignment of Listonella damsela (Love et al.) MacDonell and Colwell to the genus Photobacterium as Photobacterium damsela comb. nov. with an emended description. Int J Syst Bacteriol 41:529–534[CrossRef]
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849[CrossRef]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128[CrossRef]
UrakawaH.,
Kita-TsukamotoK.,
OhwadaK.1999; Reassessment of the taxonomic position of Vibrio iliopiscarius (Onarheim et al . 1994) and proposal for Photobacterium iliopiscarium comb. nov. Int J Syst Bacteriol 49:257–260[CrossRef]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.other authors1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]
XieC.-H.,
YokotaA.2004; Transfer of Hyphomicrobium indicum to the genus Photobacterium as Photobacterium indicum comb. nov. Int J Syst Evol Microbiol 54:2113–2116[CrossRef]
YoonJ.-H.,
KimH.,
KimS.-B.,
KimH.-J.,
KimW. Y.,
LeeS. T.,
GoodfellowM.,
ParkY.-H.1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505[CrossRef]
YoonJ.-H.,
LeeS. T.,
ParkY.-H.1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194[CrossRef]
YoonJ.-H.,
KimI.-G.,
ShinD.-Y.,
KangK. H.,
ParkY.-H.2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57[CrossRef]
YoonJ. H.,
LeeJ. K.,
KimY. O.,
OhT. K.2005; Photobacterium lipolyticum sp. nov., a bacterium with lipolytic activity isolated from the Yellow Sea in Korea. Int J Syst Evol Microbiol 55:335–339[CrossRef]