1887

Abstract

A culture (designated strain HHS02) was isolated from Chinese prawn (, O'sbeck) and determined to be a member of the genus . Strain HHS02 comprised slightly curved, rod-shaped, non-endospore-forming, Gram-negative, catalase-negative, oxidase-positive, O/129-sensitive and facultatively anaerobic cells that were motile by means of a single polar flagellum. Growth of strain HHS02 occurred in 0.5–7 % (w/v) NaCl [optimally in 1–3 % (w/v) NaCl] and between pH 7.0 and 10.0 (optimally at pH 8.0–9.0). The strain showed growth between 16 and 30 °C (optimum 20 °C). Analysis using the 16S rRNA, , , , , and gene sequences of the novel isolate revealed that the organism belonged to the genus , with ∼98, 98, 90, 88, 92, 89 and 83 % sequence similarity, respectively, with representatives of the genus . DNA–DNA hybridization experiments indicated that the novel strain was distinct from recognized species of the genus . The major fatty acid components were summed feature 3 (C 7 and/or iso-C 2-OH, 38.7 %), C (22.9 %) and C 7 (12.5 %). The G+C content of the genomic DNA was 44.4 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, it is concluded that strain HHS02 should be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HHS02 (=CGMCC 1.8461=LMG 24781).

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2010-11-01
2024-03-28
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References

  1. Alsina M., Blanch A. R. 1994; A set of keys for biochemical identification of environmental Vibrio species. J Appl Bacteriol 76:79–85 [CrossRef]
    [Google Scholar]
  2. Austin B., Austin D. A. 1989 Methods for the Microbiological Examination of Fish and Shellfish Chichester: Ellis Horwood;
    [Google Scholar]
  3. Austin B., Austin D. A. 2007 Bacterial Fish Pathogens: Diseases of Farmed and Wild Fish, 4th edn. Chichester: Springer-Praxis;
    [Google Scholar]
  4. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) 1995 Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York: Wiley;
    [Google Scholar]
  5. Beaz Hidalgo R., Cleenwerck I., Balboa S., Prado S., De Vos P., Romalde J. L. 2009; Vibrio breoganii sp. nov., a non-motile, alginolytic, marine bacterium within the Vibrio halioticoli clade. Int J Syst Evol Microbiol 59:1589–1594 [CrossRef]
    [Google Scholar]
  6. Borrego J. J., Castro D., Luque A., Paillard C., Maes P., Garcia M. T., Ventosa A. 1996; Vibrio tapetis sp. nov., the causative agent of the brown ring disease affecting cultured clams. Int J Syst Bacteriol 46:480–484 [CrossRef]
    [Google Scholar]
  7. Chang H. W., Roh S. W., Kim K. H., Nam Y. D., Jeon C. O., Oh H. M., Bae J. W. 2008; Vibrio areninigrae sp. nov., a marine bacterium isolated from black sand. Int J Syst Evol Microbiol 58:1903–1906 [CrossRef]
    [Google Scholar]
  8. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  9. Cowan S. T., Steel K. J. 1974 Manual for the Identification of Medical Bacteria, 2nd edn. Revised by S. T. Cowan Cambridge, UK: Cambridge University Press;
    [Google Scholar]
  10. Embley T. M. 1991; The linear PCR reaction: a simple and robust method for sequencing rRNA genes. Lett Appl Microbiol 13:171–174 [CrossRef]
    [Google Scholar]
  11. Farmer J. J., Janda J. M., Brenner F. W., Cameron D. N., Birkhead K. M. 2005; Genus I. Vibrio Pacini 1854, 411AL . In Bergey's Manual of Systematic Bacteriology, 2nd edn. vol 2B pp. 494–546 Edited by Brenner D. J., Krieg N. R., Staley J. T. Baltimore: The Williams & Wilkins Co;
    [Google Scholar]
  12. Gomez-Gil B., Tron-Mayén L., Roque A., Turnbull J. F., Inglis V., Guerra-Flores A. L. 1998; Species of Vibrio isolated from hepatopancreas, haemolymph and digestive tract of a population of healthy juvenile Penaeus vannamei . Aquaculture 163:1–9 [CrossRef]
    [Google Scholar]
  13. Gomez-Gil B., Fajer-Avila E., Pascual J., Macián M. C., Pujalte M. J., Garay E., Roque A. 2008; Vibrio sinaloensis sp. nov., isolated from the spotted rose snapper, Lutjanus guttatus Steindachner, 1869. Int J Syst Evol Microbiol 58:1621–1624 [CrossRef]
    [Google Scholar]
  14. Huq A., Colwell R. R. 1995; Vibrios in the marine and estuarine environments. J Mar Biotechnol 3:60–63
    [Google Scholar]
  15. Kalina G. P., Antonov A. S., Turova T. P., Grafova T. I. 1984; Allomonas enterica gen. nov., sp. nov.: deoxyribonucleic acid homology between Allomonas and some other members of the Vibrionaceae . Int J Syst Bacteriol 34:150–154 [CrossRef]
    [Google Scholar]
  16. Kumar N. R., Nair S. 2007; Vibrio rhizosphaerae sp. nov., a red-pigmented bacterium that antagonizes phytopathogenic bacteria. Int J Syst Evol Microbiol 57:2241–2246 [CrossRef]
    [Google Scholar]
  17. Liu C., Shao Z. 2005; Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55:1181–1186 [CrossRef]
    [Google Scholar]
  18. MacDonell M. T., Colwell R. R. 1985; Phylogeny of the Vibrionaceae , and recommendation for two new genera, Listonella and Shewanella . Syst Appl Microbiol 6:171–182 [CrossRef]
    [Google Scholar]
  19. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479:297–306 [CrossRef]
    [Google Scholar]
  20. Nam Y. D., Chang H. W., Park J. R., Kwon H. Y., Quan Z. X., Park Y. H., Kim B. C., Bae J. W. 2007; Vibrio litoralis sp. nov., isolated from a Yellow Sea tidal flat in Korea. Int J Syst Evol Microbiol 57:562–565 [CrossRef]
    [Google Scholar]
  21. Noguerola I., Blanch A. R. 2008; Identification of Vibrio spp. with a set of dichotomous keys. J Appl Microbiol 105:175–185 [CrossRef]
    [Google Scholar]
  22. Rameshkumar N., Fukui Y., Sawabe T., Nair S. 2008; Vibrio porteresiae sp. nov., a diazotrophic bacterium isolated from a mangrove-associated wild rice ( Porteresia coarctata Tateoka). Int J Syst Evol Microbiol 58:1608–1615 [CrossRef]
    [Google Scholar]
  23. Riquelme C. E., Jorquera M. A., Rojas A. I., Avendano R. E., Reyes N. 2001; Addition of inhibitor-producing bacteria to mass cultures of Argopecten purpuratus larvae (Lamarck, 1819. Aquaculture 192:111–119 [CrossRef]
    [Google Scholar]
  24. Sawabe T., Fujimura Y., Niwa K., Aono H. 2007a; Vibrio comitans sp. nov., Vibrio rarus sp. nov. and Vibrio inusitatus sp. nov., from the gut of the abalones Haliotis discus discus , H. gigantea , H.madaka and H. rufescens . Int J Syst Evol Microbiol 57:916–922 [CrossRef]
    [Google Scholar]
  25. Sawabe T., Kita-Tsukamoto K., Thompson F. L. 2007b; Inferring the evolutionary history of vibrios by means of multilocus sequence analysis. J Bacteriol 189:7932–7936 [CrossRef]
    [Google Scholar]
  26. Smibert R. M., Krieg N. R. 1994 Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654 Edited by Gerhardt F., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  27. Sorokin D. Y. 1992; Catenococcus thiocyclus gen. nov., sp. nov. – a new facultatively anaerobic bacterium from a near-shore sulphidic hydrothermal area. J Gen Microbiol 138:2287–2292 [CrossRef]
    [Google Scholar]
  28. Suantika G., Dhert P., Rombaut G., Vandenberghe J., De Wolf T., Sorgeloos P. 2001; The use of ozone in a high density recirculation system for rotifers. Aquaculture 201:35–49 [CrossRef]
    [Google Scholar]
  29. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  30. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  31. Thompson F. L., Hoste B., Vandemeulebroecke K., Swings J. 2001; Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism. Syst Appl Microbiol 24:520–538 [CrossRef]
    [Google Scholar]
  32. Thompson F. L., Hoste B., Thompson C. C., Goris J., Gomez-Gil B., Huys L., De Vos P., Swings J. 2002; Enterovibrio norvegicus gen. nov., sp. nov. isolated from the gut of turbot ( Scophthalmus maximus ) larvae: a new member of the family Vibrionaceae . Int J Syst Evol Microbiol 52:2015–2022 [CrossRef]
    [Google Scholar]
  33. Thompson F. L., Hoste B., Vandemeulebroecke K., Swings J. 2003; Reclassification of Vibrio hollisae as Grimontia hollisae gen. nov., comb. nov. Int J Syst Evol Microbiol 53:1615–1617 [CrossRef]
    [Google Scholar]
  34. Thompson F. L., Gevers D., Thompson C. C., Dawyndt P., Naser S., Hoste B., Munn C. B., Swings J. 2005; Phylogeny and molecular identification of vibrios on the basis of Multilocus Sequence Analysis. Appl Environ Microbiol 71:5107–5115 [CrossRef]
    [Google Scholar]
  35. Thompson F. L., Gromez-Gil B., Vasconcelos A. T. R., Sawabe T. 2007; Multilocus sequence analysis reveals that Vibrio harveyi and V. campbellii are distinct species. Appl Environ Microbiol 73:4279–4285 [CrossRef]
    [Google Scholar]
  36. Urbanczyk H., Ast J. C., Higgins M. J., Carson J., Dunlap P. V. 2007; Reclassification of Vibrio fischeri , Vibrio logei , Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen.nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio salmonicida comb. nov. and Aliivibrio wodanis comb. nov. Int J Syst Evol Microbiol 57:2823–2829 [CrossRef]
    [Google Scholar]
  37. Verschuere L., Rombaut G., Sorgeloos P., Verstraete W. 2000; Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev 64:655–671 [CrossRef]
    [Google Scholar]
  38. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  39. Xie C. H., Yokota A. 2003; Phylogenetic analysis of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [CrossRef]
    [Google Scholar]
  40. Yoshizawa S., Wada M., Kita-Tsukamoto K., Ikemoto E., Yokota A., Kogure K. 2009; Vibrio azureus sp. nov., a luminous marine bacterium isolated from seawater. Int J Syst Evol Microbiol 59:1645–1649 [CrossRef]
    [Google Scholar]
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