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Volume 60, Issue 11

sp. nov., isolated from the digestive tract of the Chinese prawn ( O'sbeck) Free

Abstract

A culture (designated strain HHS02) was isolated from Chinese prawn (, O'sbeck) and determined to be a member of the genus . Strain HHS02 comprised slightly curved, rod-shaped, non-endospore-forming, Gram-negative, catalase-negative, oxidase-positive, O/129-sensitive and facultatively anaerobic cells that were motile by means of a single polar flagellum. Growth of strain HHS02 occurred in 0.5–7 % (w/v) NaCl [optimally in 1–3 % (w/v) NaCl] and between pH 7.0 and 10.0 (optimally at pH 8.0–9.0). The strain showed growth between 16 and 30 °C (optimum 20 °C). Analysis using the 16S rRNA, , , , , and gene sequences of the novel isolate revealed that the organism belonged to the genus , with ∼98, 98, 90, 88, 92, 89 and 83 % sequence similarity, respectively, with representatives of the genus . DNA–DNA hybridization experiments indicated that the novel strain was distinct from recognized species of the genus . The major fatty acid components were summed feature 3 (C 7 and/or iso-C 2-OH, 38.7 %), C (22.9 %) and C 7 (12.5 %). The G+C content of the genomic DNA was 44.4 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, it is concluded that strain HHS02 should be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HHS02 (=CGMCC 1.8461=LMG 24781).

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Keyword(s): MLSA, multilocus sequence analysis and O/129, 2,4-diamino-6,7-diisopropyl pteridine phosphate salt
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2010-11-01
2023-09-30
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Vibrio atypicus sp. nov., isolated from the digestive tract of the Chinese prawn (Penaeus chinensis O'sbeck)
Int J Syst Evol Microbiol 60, 2517 (2010); https://doi.org/10.1099/ijs.0.016915-0
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