1887

Abstract

Two novel bacterial strains were isolated from tropical rain forest soil from Thailand. Strains A-T 0875 and A-T 1383 stained Gram-positive and were filamentous bacteria that developed cylindrical sporangia containing four oval- to rod-shaped spores at the ends of short sporangiophores on branched aerial mycelium. The cell-wall peptidoglycan contained -diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; whole-cell hydrolysates contained rhamnose, madurose, glucose, galactose and 3--methylmannose as whole-cell sugars. The predominant menaquinone was MK-9(H). Mycolic acids were not detected. The diagnostic phospholipid was phosphatidylethanolamine. The predominant cellular fatty acids were iso-C and 10-methyl-C. For both strains, the GC content of the genomic DNA was 71 mol%. Phenotypic and chemotaxonomic analyses showed that the characteristics of the two isolates were typical of members of the genus . Furthermore, 16S rRNA gene sequence analysis also indicated that the strains belonged to the genus but as representatives of two novel species. Following an evaluation of our phenotypic, chemotaxonomic and genotypic studies, two novel species are proposed, sp. nov. (type strain A-T 0875=BCC 24832=NBRC 104272) and sp. nov. (type strain A-T 1383=BCC 26045=NBRC 104273).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.016899-0
2010-09-01
2020-09-30
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/9/2076.html?itemId=/content/journal/ijsem/10.1099/ijs.0.016899-0&mimeType=html&fmt=ahah

References

  1. Arai T. 1975 Culture Media for Actinomycetes Tokyo: The Society for Actinomycetes Japan;
    [Google Scholar]
  2. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. 1981; Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli . J Mol Biol 148:107–127 [CrossRef]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  7. Hayakawa M., Sadakata T., Kajiura T., Nonomura H. 1991; New methods for the highly selective isolation of Micromonospora and Microbispora from soil. J Ferment Bioeng 72:320–326 [CrossRef]
    [Google Scholar]
  8. Itoh T., Kudo T., Parenti F., Seino A. 1989; Amended description of the genus Kineosporia , based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 39:168–173 [CrossRef]
    [Google Scholar]
  9. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  10. Katsura K., Kawasaki H., Potacharoen W., Saono S., Seki T., Yamada Y., Uchimura T., Komagata K. 2001; Asaia siamensis sp. nov., an acetic acid bacterium in the α-Proteobacteria . Int J Syst Evol Microbiol 51:559–563
    [Google Scholar]
  11. Kawamoto I., Oka T., Nara T. 1981; Cell wall composition of Micromonospora olivoasterospora , Micromonospora sagamiensis , and related organisms. J Bacteriol 146:527–534
    [Google Scholar]
  12. Kawasaki H., Hoshino Y., Hirata A., Yamasato K. 1993; Is intracytoplasmic membrane structure a generic criterion? It does not coincide with phylogenetic interrelationships among photosynthetic purple non-sulfur bacteria. Arch Microbiol 160:358–362
    [Google Scholar]
  13. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  14. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics ( Society for Applied Bacteriology Technical Series, vol 20) pp 173–199 Edited by Goodfellow M., Minnikin D. E. New York: Academic Press;
    [Google Scholar]
  15. Kudo T., Itoh T., Miyadoh S., Shomura T., Seino A. 1993; Herbidospora gen. nov., a new genus of the family Streptosporangiaceae Goodfellow et al. 1990. Int J Syst Bacteriol 43:319–328 [CrossRef]
    [Google Scholar]
  16. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  17. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  18. Mikami H., Ishida Y. 1983; Post-column fluorometric detection of reducing sugar in high-performance liquid chromatography using arginine. Bunseki Kagaku 32:E207–E210 [CrossRef]
    [Google Scholar]
  19. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  20. Nonomura H., Ohara Y. 1957; Distribution of Actinomycetes in the soil. II. Microbispora ,a new genus of the Streptomycetaceae . J Ferment Technol 35:307–311
    [Google Scholar]
  21. Runmao H., Guizhen W., Junying L. 1993; A new genus of actinomycetes, Planotetraspora gen. nov. Int J Syst Bacteriol 43:468–470 [CrossRef]
    [Google Scholar]
  22. Saito H., Miura K. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629 [CrossRef]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101: Newark, DE: MIDI, Inc;
    [Google Scholar]
  25. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  26. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  27. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  28. Suriyachadkun C., Chunhametha S., Thawai C., Tamura T., Potacharoen W., Kirtikara K., Sanglier J. J. 2009; Planotetraspora thailandica sp. nov., isolated from soil in Thailand. Int J Syst Evol Microbiol 59:992–997 [CrossRef]
    [Google Scholar]
  29. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  30. Tamura T., Sakane T. 2004; Planotetraspora silvatica sp. nov. and emended description of the genus Planotetraspora . Int J Syst Evol Microbiol 54:2053–2056 [CrossRef]
    [Google Scholar]
  31. Tamura T., Nakagaito Y., Nishii T., Hasegawa T., Stackebrandt E., Yokota A. 1994 A new genus of the order Actinomycetales , Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb.nov. and Couchioplanescaeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44, 193–203 [CrossRef]
  32. Tamura T., Suzuki S., Hatano K. 2000; Acrocarpospora gen. nov., a new genus of the order Actinomycetales . Int J Syst Evol Microbiol 50:1163–1171 [CrossRef]
    [Google Scholar]
  33. Thiemann J. E., Pagani H., Beretta G. 1968; A new genus of the Actinomycetales : Microtetraspora gen. nov. J Gen Microbiol 50:295–303 [CrossRef]
    [Google Scholar]
  34. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [CrossRef]
    [Google Scholar]
  35. Verlander C. P. 1992; Detection of horseradish peroxidase by colorimetry. In Nonisotopic DNA Probe Techniques pp 185–201 Edited by Kricka L. J. New York: Academic Press;
    [Google Scholar]
  36. Williams S. T., Cross T. 1971; Actinomycetes. Methods Microbiol 4:295–334
    [Google Scholar]
  37. Yamada Y., Katsura K., Kawasaki K., Widyastuti Y., Saono S., Seki T., Uchimura T., Komagata K. 2000; Asaia bogorensis gen. nov., sp. nov. an unusual acetic acid bacterium in the α-Proteobacteria . Int J Syst Evol Microbiol 50:823–829 [CrossRef]
    [Google Scholar]
  38. Yukphan P., Potacharoen W., Tanasupawat S., Tanticharoen M., Yamada Y. 2004; Asaia krungthepensis sp. nov., an acetic acid bacterium in the α-Proteobacteria . Int J Syst Evol Microbiol 54:313–316 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.016899-0
Loading
/content/journal/ijsem/10.1099/ijs.0.016899-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error