1887

Abstract

An actinomycete, designated CPCC 202699, was isolated from soil in Qinghai province, China, and its taxonomic status was established. Strain CPCC 202699 contained -diaminopimelic acid in its peptidoglycan, arabinose and galactose as the diagnostic sugars in whole-cell hydrolysates, a phospholipid pattern consisting mainly of phosphatidylethanolamine, phosphatidylmethylethanolamine and phosphatidylcholine, MK-9(H) as the predominant menaquinone and iso-C (18.8 %), iso-C (18.1 %), iso-C (14.2 %), C 9 (10.9 %) and C 9 (10.3 %) as the major fatty acids. In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 202699 formed a separate branch within the genus . However, strain CPCC 202699 showed low 16S rRNA gene sequence similarities (<97.0 %) with type strains of species with validly published names in the genus . On the basis of genotypic and phenotypic differences from the closest phylogenetic neighbours of strain CPCC 202699, a novel species, sp. nov., is proposed. The type strain is CPCC 202699 (=DSM 45285 =KCTC 19581 =CCM 7627).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.016865-0
2010-09-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/9/2124.html?itemId=/content/journal/ijsem/10.1099/ijs.0.016865-0&mimeType=html&fmt=ahah

References

  1. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  2. Chun, J., Lee, J. H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  3. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  4. Embley, T. M., Smida, J. & Stackebrandt, E. ( 1988; ). The phylogeny of mycolate-less wall chemotype IV actinomycetes and description of Pseudonocardiaceae fam. nov. Syst Appl Microbiol 11, 44–52.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  6. Groth, I., Schumann, P., Rainey, F. A., Martin, K., Schuetze, B. & Augsten, K. ( 1997; ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47, 1129–1133.[CrossRef]
    [Google Scholar]
  7. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  8. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  9. Kelly, K. L. ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC. : US Government Printing Office.
    [Google Scholar]
  10. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  11. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge. : Cambridge University Press.
    [Google Scholar]
  12. Labeda, D. P., Price, N. P., Tan, G. Y. A., Goodfellow, M. & Klenk, H.-P. ( 2010; ). Emended description of the genus Actinokineospora Hasegawa 1988 and transfer of Amycolatopsis fastidiosa Henssen et al. 1987 as Actinokineospora fastidiosa comb. nov. Int J Syst Evol Microbiol 60, 1444–1449.[CrossRef]
    [Google Scholar]
  13. Lechevalier, M. P. & Lechevalier, H. A. ( 1980; ). The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy (SIM Special Publication no. 6), pp. 227–291. Edited by Dietz, A. & Thayer, D. W.. Fairfax, VA. : Society for Industrial Microbiology.
    [Google Scholar]
  14. Lechevalier, M. P., Prauser, H., Labeda, D. P. & Ruan, J.-S. ( 1986; ). Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov. Int J Syst Bacteriol 36, 29–37.[CrossRef]
    [Google Scholar]
  15. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  16. Minnikin, D. E., Alshamaony, L. & Goodfellow, M. ( 1975; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88, 200–204.[CrossRef]
    [Google Scholar]
  17. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  18. Rosselló-Mora, R. & Amann, R. ( 2001; ). The species concept for prokaryotes. FEMS Microbiol Rev 25, 39–67.[CrossRef]
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16.
    [Google Scholar]
  21. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  22. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  23. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  24. Tan, G. Y., Ward, A. C. & Goodfellow, M. ( 2006; ). Exploration of Amycolatopsis diversity in soil using genus-specific primers and novel selective media. Syst Appl Microbiol 29, 557–569.[CrossRef]
    [Google Scholar]
  25. Warwick, S., Bowen, T., McVeigh, H. & Embley, T. M. ( 1994; ). A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the genera Amycolata and Pseudoamycolata in an emended genus Pseudonocardia. Int J Syst Bacteriol 44, 293–299.[CrossRef]
    [Google Scholar]
  26. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J. ( 1983; ). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813.
    [Google Scholar]
  27. Yuan, L. J., Zhang, Y. Q., Guan, Y., Wei, Y. Z., Li, Q. P., Yu, L. Y., Li, W. J. & Zhang, Y. Q. ( 2008; ). Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil. Int J Syst Evol Microbiol 58, 1180–1185.[CrossRef]
    [Google Scholar]
  28. Zhou, Z. H., Liu, Z. H., Qian, Y. D., Kim, S. B. & Goodfellow, M. ( 1998; ). Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48, 53–58.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.016865-0
Loading
/content/journal/ijsem/10.1099/ijs.0.016865-0
Loading

Data & Media loading...

Supplements

Two-dimensional TLC of phosphate-containing lipids of strain CPCC 202699 , with molybdenum blue as the spray reagent. GluNU/NPG, Glucosamine-containing phospholipids; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PIDM, phosphatidylinositol dimannoside; PIM, phosphatidylinositol mannoside; PME, phosphatidylmethylethanolamine.

IMAGE

Cellular fatty acid contents of strain CPCC 202699 and related type strains of the genus . [PDF](43 KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error