1887

Abstract

A Gram-negative-staining, non-motile, non-spore-forming and strictly aerobic bacterial strain, SC35, was isolated from tidal flat sediment collected from the South Sea, Korea, and subjected to a taxonomic study using a polyphasic approach. The organism grew optimally at 20–30 °C and with 1–2 % (w/v) NaCl. Strain SC35 contained ubiquinone-8 as the predominant respiratory lipoquinone and C 9 as the major fatty acid. The DNA GC content was 48.5 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain SC35 formed a lineage within the genus (94.3–96.5 % sequence similarity), forming a distinct branch in a clade also containing NIBH P2K6 and SW-238. On the basis of phenotypic and phylogenetic data, strain SC35 (=KCTC 22503=JCM 16343) was placed in the genus as the type strain of a novel species, for which the name sp. nov. is proposed.

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2010-07-01
2019-09-15
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References

  1. Bakermans, C., Ayala-del-Río, H. L., Ponder, M. A., Vishnivetskaya, T., Gilichinsky, D., Thomashow, M. F. & Tiedje, J. M. ( 2006; ). Psychrobacter cryohalolentis sp. nov. and Psychrobacter arcticus sp. nov., isolated from Siberian permafrost. Int J Syst Evol Microbiol 56, 1285–1291.[CrossRef]
    [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  3. Bowman, J. P., Cavanagh, J., Austin, J. J. & Sanderson, K. ( 1996; ). Novel Psychrobacter species from Antarctic ornithogenic soils. Int J Syst Bacteriol 46, 841–848.[CrossRef]
    [Google Scholar]
  4. Bowman, J. P., Nichols, D. S. & McMeekin, T. A. ( 1997; ). Psychrobacter glacincola sp. nov., a halotolerant, psychrophilic bacterium isolated from Antarctic sea ice. Syst Appl Microbiol 20, 209–215.[CrossRef]
    [Google Scholar]
  5. Bozal, N., Montes, J., Tudela, E. & Guinea, J. ( 2003; ). Characterization of several Psychrobacter strains isolated from Antarctic environments and description of Psychrobacter luti sp. nov. and Psychrobacter fozii sp. nov. Int J Syst Evol Microbiol 53, 1093–1100.[CrossRef]
    [Google Scholar]
  6. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  7. Chun, J., Bae, K. S., Moon, E. Y., Jung, S. O., Lee, H. K. & Kim, S. J. ( 2000; ). Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913.
    [Google Scholar]
  8. CLSI ( 2003; ). Performance Standards for Antimicrobial Disk Susceptibility Tests, 8th edn. Approved Standard M2–A8. Wayne, PA: Clinical Laboratory Standards Institute.
  9. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  10. Denner, E. B. M., Mark, B., Busse, H.-J., Turkiewicz, M. & Lubitz, W. ( 2001; ). Psychrobacter proteolyticus sp. nov., a psychrotrophic, halotolerant bacterium isolated from the Antarctic krill Euphausia superba Dana, excreting a cold-adapted metalloprotease. Syst Appl Microbiol 24, 44–53.[CrossRef]
    [Google Scholar]
  11. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  12. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  13. González, C. J., Santos, J. A., García-López, M. L. & Otero, A. ( 2000; ). Psychrobacters and related bacteria in freshwater fish. J Food Prot 63, 315–321.
    [Google Scholar]
  14. Heuchert, A., Glöckner, F. O., Amann, R. & Fischer, U. ( 2004; ). Psychrobacter nivimaris sp. nov., a heterotrophic bacterium attached to organic particles isolated from the South Atlantic (Antarctica). Syst Appl Microbiol 27, 399–406.[CrossRef]
    [Google Scholar]
  15. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  16. Jung, S.-Y., Lee, M.-H., Oh, T.-K., Park, Y.-H. & Yoon, J.-H. ( 2005; ). Psychrobacter cibarius sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 55, 577–582.[CrossRef]
    [Google Scholar]
  17. Juni, E. & Heym, G. A. ( 1986; ). Psychrobacter immobilis gen. nov., sp. nov.: genospecies composed of Gram-negative, aerobic, oxidase-positive coccobacilli. Int J Syst Bacteriol 36, 388–391.[CrossRef]
    [Google Scholar]
  18. Kämpfer, P., Albrecht, A., Buczolits, S. & Busse, H.-J. ( 2002; ). Psychrobacter faecalis sp. nov., a new species from a bioaerosol originating from pigeon faeces. Syst Appl Microbiol 25, 31–36.[CrossRef]
    [Google Scholar]
  19. Kovacs, N. ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178, 703
    [Google Scholar]
  20. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  21. Maruyama, A., Honda, D., Yamamoto, H., Kitamura, K. & Higashihara, T. ( 2000; ). Phylogenetic analysis of psychrophilic bacteria isolated from the Japan Trench, including a description of the deep-sea species Psychrobacter pacificensis sp. nov. Int J Syst Evol Microbiol 50, 835–846.[CrossRef]
    [Google Scholar]
  22. MIDI ( 1999; ). Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE: MIDI Inc.
  23. Richard, C. & Kiredjian, M. ( 1995; ). Laboratory Methods for the Identification of Strictly Aerobic Gram-negative Bacilli. Paris: Institut Pasteur.
  24. Romanenko, L. A., Schumann, P., Rohde, M., Lysenko, A. M., Mikhailov, V. V. & Stackebrandt, E. ( 2002; ). Psychrobacter submarinus sp. nov. and Psychrobacter marincola sp. nov., psychrophilic halophiles from marine environments. Int J Syst Evol Microbiol 52, 1291–1297.[CrossRef]
    [Google Scholar]
  25. Romanenko, L. A., Lysenko, A. M., Rohde, M., Mikhailov, V. V. & Stackebrandt, E. ( 2004; ). Psychrobacter maritimus sp. nov. and Psychrobacter arenosus sp. nov., isolated from coastal sea ice and sediments of the Sea of Japan. Int J Syst Evol Microbiol 54, 1741–1745.[CrossRef]
    [Google Scholar]
  26. Romanenko, L. A., Tanlka, N., Frolova, G. M. & Mikhailov, V. V. ( 2009; ). Psychrobacter fulvigenes sp. nov., isolated from a marine crustacean from the Sea of Japan. Int J Syst Evol Microbiol 59, 1480–1489.[CrossRef]
    [Google Scholar]
  27. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  28. Shivaji, S., Reddy, G. S. N., Raghavan, P. U. M., Sarita, N. B. & Deille, D. ( 2004; ). Psychrobacter salsus sp. nov. and Psychrobacter adeliensis sp. nov. isolated from fast ice from Adelie Land, Antarctica. Syst Appl Microbiol 27, 628–635.[CrossRef]
    [Google Scholar]
  29. Shivaji, S., Reddy, G. S. N., Suresh, K., Gupta, P., Chintalapati, S., Schumann, P., Stackebrandt, E. & Matsumoto, G. I. ( 2005; ). Psychrobacter vallis sp. nov. and Psychrobacter aquaticus sp. nov., from Antarctica. Int J Syst Evol Microbiol 55, 757–762.[CrossRef]
    [Google Scholar]
  30. Skerman, V. B. D. ( 1967; ). A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  31. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  32. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  33. Swofford, D. L. ( 1998; ). Phylogenetic analysis using parsimony (paup), version 4. Sunderland, MA: Sinauer Associates.
  34. Vela, A. I., Collins, M. D., Latre, M. V., Mateos, A., Moreno, M. A., Hutson, R., Domínguez, L. & Fernández-Garayzábal, J. F. ( 2003; ). Psychrobacter pulmonis sp. nov., isolated from the lungs of lambs. Int J Syst Evol Microbiol 53, 415–419.[CrossRef]
    [Google Scholar]
  35. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  36. Yamaguchi, S. & Yokoe, M. ( 2000; ). A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. Appl Environ Microbiol 66, 3337–3343.[CrossRef]
    [Google Scholar]
  37. Yoon, J.-H., Kang, K. H. & Park, Y.-H. ( 2003; ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53, 449–454.[CrossRef]
    [Google Scholar]
  38. Yoon, J.-H., Lee, C.-H., Yeo, S.-H. & Oh, T.-K. ( 2005a; ). Psychrobacter aquimaris sp. nov. and Psychrobacter namhaensis sp. nov., isolated from sea water of the South Sea in Korea. Int J Syst Evol Microbiol 55, 1007–1013.[CrossRef]
    [Google Scholar]
  39. Yoon, J.-H., Yeo, S.-H., Oh, T.-K. & Park, Y.-H. ( 2005b; ). Psychrobacter alimentarius sp. nov., isolated from squid jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 55, 171–176.[CrossRef]
    [Google Scholar]
  40. Yoon, J. H., Lee, C. H., Kang, S. J. & Oh, T. K. ( 2005c; ). Psychrobacter celer sp. nov., isolated from sea water of the South Sea in Korea. Int J Syst Evol Microbiol 55, 1885–1890.[CrossRef]
    [Google Scholar]
  41. Yumoto, I., Hirota, K., Sogabe, Y., Nodasaka, Y., Yokota, Y. & Hoshino, T. ( 2003; ). Psychrobacter okhotskensis sp. nov., a lipase-producing facultative psychrophile isolated from the coast of the Okhotsk Sea. Int J Syst Evol Microbiol 53, 1985–1989.[CrossRef]
    [Google Scholar]
  42. Yumoto, I., Hirota, K., Kimoto, H., Nodasaka, Y., Matsuyama, H. & Yoshimune, K. ( 2009; ). Psychrobacter piscatorii sp. nov., psychrotolerant bacterium exhibiting high catalase activity isolated from oxidative environment. Int J Syst Evol Microbiol 60, 205–208.
    [Google Scholar]
  43. ZoBell, C. E. ( 1941; ). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4, 42–75.
    [Google Scholar]
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