1887

Abstract

We isolated several strains from various clinical samples (five samples of blood, four of intra-abdominal pus and one of infected soft tissue) that were anaerobic, motile or non-motile and Gram-positive rods. Some of the strains formed spores. Phylogenetic analysis of the 16S rRNA gene sequence showed that these organisms could be placed within clostridial cluster IV as defined by Collins [(1994). , 812–826] and shared more than 99 % sequence similarity with DSM 6740 and DSM 4000. Together, they formed a distinct cluster, with ATCC 29799 branching off from this line of descent with sequence similarities of 97.1–97.4 %. The next nearest neighbours of these organisms were , , and , with sequence similarities to the respective type strains of 93.1–93.4, 91.2–91.4, 89.8–90 and 88.7–89.3 %. On the basis of biochemical properties, phylogenetic position, DNA G+C content and DNA–DNA hybridization, it is proposed to unify and in a new genus as gen. nov., comb. nov., with the type strain Prévot S1 (=ATCC 29863 =VPI 0310 =DSM 4000), and to reassign to gen. nov., comb. nov., with the type strain CCUG 15402A (=ATCC 29799 =VPI R2-29-1).

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2010-03-01
2019-10-16
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DPH fluorescence-quenching test. The test is based on the ability of flavonoids to quench DPH fluorescence. Each tube contains DPH and quercetin unless indicated. 1, K-12 (quercetin non-degrading strain); 2, AIP 201.04; 3, DSM 6740 ; 4, AIP 165.06; 5, AIP 162.06; 6, DSM 4000 ; 7, AIP 029.07; 8, AIP 028.07; 9, DPH plus quercetin only (negative control); 10, DPH only (positive control).

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PCR amplification of fragments. Lanes: M, 100 bp ladder size marker (Invitrogen); 1, DSM 4000 ; 2, DSM 6740 ; 3, AIP 028.07; 4, AIP 029.07; 5, K-12 (non-spore-forming species); 6, ATCC 13124 (asporogenic species on regular culture media); 7, negative control.

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