Nine Gram-negative, catalase- and oxidase-positive, coccus-shaped bacteria were isolated from pigs affected by different pathological processes. Phenotypic and genotypic methods were adopted to determine the relationships of these new isolates to recognized species of the genus Moraxella. Analysis of the 16S rRNA gene sequences demonstrated that the clinical isolates represented a new lineage within the genus Moraxella. The isolates were closely related to Moraxella cuniculi and Moraxella pluranimalium with 16S rRNA gene sequence similarities of 98.1 % and 99.1 %, respectively. The isolates displayed DNA–DNA relative binding ratios of 74 % to each other, but distinctly lower levels of DNA–DNA hybridization were observed with phylogenetically closely related moraxellae (<32 %). The new isolates could be distinguished from all other recognized species of the genus Moraxella by physiological and biochemical tests. On the basis of the phenotypic and molecular data, the nine new isolates from pigs represent a novel species within the genus Moraxella, for which the name Moraxella porci sp. nov. is proposed. The type strain is SN9-4MT (=CECT 7294T=CCUG 54912T).
AngelosJ. A.,
SpinksP. Q.,
BallL. M.,
GeorgeL. W.2007; Moraxella bovoculi sp. nov., isolated from calves with infectious bovine keratoconjunctivitis. Int J Syst Evol Microbiol 57:789–795[CrossRef]
ArahalD. R.,
GarcíaM. T.,
VargasC.,
CanovasD.,
NietoJ. J.,
VentosaA.2001; Chromohalobacter salexigens sp. nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174. Int J Syst Evol Microbiol 51:1457–1462
JohnsonJ. L.1994; Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology. pp 655–681 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
JuniE.,
BøvreK.2005; Genus I. Moraxella Lwoff 1939, 173, emend. Henriksen and Bøvre 1968, 391VP. In Bergey's Manual of Systematic Bacteriology, 2nd edn. vol 2B pp 417–425 Edited by
BrennerD. J.,
KriegN. R.,
StaleyJ. T.,
GarrityG. M.
New York: Springer;
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120[CrossRef]
KodjoA.,
TønjumT.,
RichardY.,
BøvreK.1995; Moraxella caprae sp. nov., a new member of the classical moraxellae. Int J Syst Bacteriol 45:467–471[CrossRef]
LavinS.,
LastrasM. E.,
MarcoI.,
CabañesF. X.2000; Report of a case of bronchopneumonia associated with Moraxella bovis isolation in a chamois ( Rupicrapa pyrenaica ). J Vet Med 47:225–227[CrossRef]
MarmurJ.,
DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118[CrossRef]
VelaA. I.,
CollinsM. D.,
LawsonP. A.,
GarcíaN.,
DomínguezL.,
Fernández-GarayzábalJ. F.2005; Uruburuella suis gen. nov., sp. nov., isolated from clinical specimens of pigs. Int J Syst Evol Microbiol 55:643–647[CrossRef]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.other authors1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]