1887

Abstract

Strain YSM-123 was isolated from commercial salt made from Japanese seawater in Niigata prefecture. Optimal NaCl and Mg concentrations for growth were 4.0–4.5 M and 5 mM, respectively. The isolate was a mesophilic and slightly alkaliphilic haloarchaeon, whose optimal growth temperature and pH were 37 °C and pH 8.0–9.0. Phylogenetic analysis based on 16S rRNA gene sequence analysis suggested that strain YSM-123 is a member of the phylogenetic group defined by the family , but there were low similarities to type strains of other genera of this family (≤90 %); for example, (similarity <89 %), (<89 %), (<89 %), (<90 %), (<90 %), (<90 %), (<90 %), (<90 %) and (<90 %). The G+C content of the DNA was 63 mol%. Polar lipid analysis revealed the presence of phosphatidylglycerol, phosphatidylglycerophosphate methyl ester, disulfated diglycosyl diether and an unknown glycolipid. On the basis of the data presented, we propose that strain YSM-123 should be placed in a new genus and species, gen. nov., sp. nov. The type strain of is strain YSM-123 (=JCM 16328 =CECT 7565).

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2010-11-01
2019-10-22
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vol. , part 11, pp. 2529 - 2534

TLC of polar lipids of strain YSM-123 and reference haloarchaea. Circled spots are glycolipids. Lanes: 1, JCM 8978 ; 2, strain YSM-123 ; 3, JCM 9576 ; 4, JCM 11222 . PG, Phosphatidylglycerol; PGP-Me, phosphatidylglycerophosphate methyl ester; S -DGD, disulfated diglycosyl diether; S-TGD, sulfated triglycosyl diether.



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