1887

Abstract

Three teleomorphic ascomycetous yeast isolates (TA11TR-1, TA11TR-4 and TA11TR-6) from orchard soil from Tai'an, Shandong province, China, were shown to represent a novel species within the genus based on phenotypic characterization and sequence analyses of the 18S rRNA gene, internal transcribed spacer (ITS) regions and 26S rDNA gene D1/D2 domain. The name sp. nov. (type strain TA11TR-1 =AS 2.4160 =CBS 11405) is proposed. sp. nov. clustered in a branch together with , and the complex with moderate bootstrap support in the neighbour-joining tree reconstructed from combined 18S and D1/D2 sequences. The novel species possessed unusual ITS 1 (338 bp) and ITS 2 (488 bp) sequences. The total length of the ITS–5.8S rDNA gene region of the species was 983 bp, being much longer than those of other ascomycetous yeast species described so far.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.016501-0
2010-06-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/6/1473.html?itemId=/content/journal/ijsem/10.1099/ijs.0.016501-0&mimeType=html&fmt=ahah

References

  1. Bai, F. Y., Zhao, J. H., Takashima, M., Jia, J. H., Boekhout, T. & Nakase, T. ( 2002; ). Reclassification of the Sporobolomyces roseus and the Sporidiobolus pararoseus complexes, with the description of Sporobolomyces phaffii sp. nov. Int J Syst Evol Microbiol 52, 2309–2314.[CrossRef]
    [Google Scholar]
  2. Droby, S. ( 2006; ). Improving quality and safety of fresh fruits and vegetables after harvest by the use of biocontrol agents and natural materials. Acta Hortic 709, 45–52.
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Janisiewicz, W. J. ( 1996; ). Ecological diversity, niche overlap, and coexistence of antagonists used in developing mixtures for biocontrol of postharvest diseases of apples. Phytopathology 86, 473–479.[CrossRef]
    [Google Scholar]
  5. Janisiewicz, W. J. & Korsten, L. ( 2002; ). Biological control of postharvest diseases of fruits. Annu Rev Phytopathol 40, 411–441.[CrossRef]
    [Google Scholar]
  6. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  7. Kurtzman, C. P., Robnett, C. J., Ward, J. M., Brayton, C., Gorelick, P. & Walsh, T. J. ( 2005; ). Multigene phylogenetic analysis of pathogenic Candida species in the Kazachstania (Arxiozyma) telluris complex and description of their ascosporic states as Kazachstania bovina sp. nov., K. heterogenica sp. nov., K. pintolopesii sp. nov., and K. slooffiae sp. nov. J Clin Microbiol 43, 101–111.[CrossRef]
    [Google Scholar]
  8. Lima, G., De Curtis, F., Piedimonte, D., Spina, A. M. & De Cicco, V. ( 2006; ). Integration of biocontrol yeast and thiabendazole protects stored apples from fungicide sensitive and resistant isolates of Botrytis cinerea. Postharvest Biol Technol 40, 301–307.[CrossRef]
    [Google Scholar]
  9. Lu, H. Z., Cai, Y., Wu, Z. W., Jia, J. H. & Bai, F. Y. ( 2004; ). Kazachstania aerobia sp. nov., a novel ascomycetous yeast species from aerobically deteriorating corn silage. Int J Syst Evol Microbiol 54, 2431–2435.[CrossRef]
    [Google Scholar]
  10. Makimura, K., Murayama, Y. S. & Yamaguchi, H. ( 1994; ). Detection of a wide range of medically important fungi by the polymerase chain reaction. J Med Microbiol 40, 358–364.[CrossRef]
    [Google Scholar]
  11. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  12. Sansone, G., Rezza, I., Calvente, V., Benuzzi, D. & Sanz de Tosetti, M. I. ( 2005; ). Control of Botrytis cinerea strains resistant to iprodione in apple with rhodotorulic acid and yeasts. Postharvest Biol Technol 35, 245–251.[CrossRef]
    [Google Scholar]
  13. Sharma, R. R., Singh, D. & Singh, R. ( 2009; ). Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: a review. Biol Control 50, 205–221.[CrossRef]
    [Google Scholar]
  14. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  15. Wang, Q. M., Bai, F. Y., Zhao, J. H. & Jia, J. H. ( 2003; ). Dioszegia changbaiensis sp. nov., a basidiomycetous yeast species isolated from northeast China. J Gen Appl Microbiol 49, 295–299.[CrossRef]
    [Google Scholar]
  16. Wilson, C. L., Wisniewski, M. E., Droby, S. & Chalutz, E. ( 1993; ). A selection strategy for microbial antagonists to control postharvest diseases of fruits and vegetables. Sci Hortic (Amsterdam) 53, 183–189.[CrossRef]
    [Google Scholar]
  17. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.016501-0
Loading
/content/journal/ijsem/10.1099/ijs.0.016501-0
Loading

Data & Media loading...

Supplements

vol. , part 6, pp. 1473 - 1476

. Electrophoretic karyotypes of strains (1) TA11TR-1 , (2) TA11TR-6 and (3) TA11TR-4.



IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error