sp. nov., isolated from the surfaces of two French red smear soft cheeses Free

Abstract

Three Gram-negative, rod-shaped, catalase- and oxidase-positive, facultatively anaerobic and motile bacteria, strains WS 4538, WS 4539 and WS 4540, were isolated from the surfaces of two fully ripened French red smear soft cheeses. Based on 16S rRNA gene sequence similarity, all three strains were shown to belong to the genus . They are most closely related to S-1 (96.3 % similarity) and MANO22D (95.9 %). DNA–DNA hybridization confirmed that all three isolates belong to the same species and clearly separated strain WS 4539 from DSM 19141 (38–42 % relatedness) and DSM 17657 (28–37 %). In contrast to their nearest relatives, the strains exhibited -galactosidase and aesculin hydrolase activities. A 14 bp insertion in the 16S rRNA gene sequence forms an elongated structure at helix 10 in the rRNA molecule and provides a tool for PCR-based identification of the novel species. Partial sequences of the housekeeping genes , , and supported the conclusion that the three isolates constitute a separate species within the genus . The name sp. nov. is proposed for the novel taxon. Strain WS 4539 (=DSM 22364 =LMG 25240; DNA G+C content 41.8 mol%) is the type strain and WS 4540 (=DSM 22378 =LMG 25241) is a reference strain.

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2010-08-01
2024-03-29
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