1887

Abstract

Three Gram-negative, rod-shaped, catalase- and oxidase-positive, facultatively anaerobic and motile bacteria, strains WS 4538, WS 4539 and WS 4540, were isolated from the surfaces of two fully ripened French red smear soft cheeses. Based on 16S rRNA gene sequence similarity, all three strains were shown to belong to the genus . They are most closely related to S-1 (96.3 % similarity) and MANO22D (95.9 %). DNA–DNA hybridization confirmed that all three isolates belong to the same species and clearly separated strain WS 4539 from DSM 19141 (38–42 % relatedness) and DSM 17657 (28–37 %). In contrast to their nearest relatives, the strains exhibited -galactosidase and aesculin hydrolase activities. A 14 bp insertion in the 16S rRNA gene sequence forms an elongated structure at helix 10 in the rRNA molecule and provides a tool for PCR-based identification of the novel species. Partial sequences of the housekeeping genes , , and supported the conclusion that the three isolates constitute a separate species within the genus . The name sp. nov. is proposed for the novel taxon. Strain WS 4539 (=DSM 22364 =LMG 25240; DNA G+C content 41.8 mol%) is the type strain and WS 4540 (=DSM 22378 =LMG 25241) is a reference strain.

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2010-08-01
2024-10-09
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References

  1. Baumann P., Baumann L. 1981; The marine gram-negative eubacteria: genera Photobacterium , Beneckea , Alteromonas , Pseudomonas , and Alcaligenes . In The Prokaryotes. A Handbook on Habitats, Isolation and Identification of Bacteria pp 1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. New York: Springer;
    [Google Scholar]
  2. Baumann P., Baumann L. 1984; Genus II. Photobacterium Beijerinck 1889, 401AL . In Bergey's Manual of Systematic Bacteriology vol. 1 pp 539–545 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Baumann P., Schubert R. H. W. 1984; Genus II. Vibrionaceae Veron 1965, 5245AL . In Bergey's Manual of Systematic Bacteriology vol. 1 pp 516–517 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Browne-Silva J., Nishiguchi M. K. 2008; Gene sequences of the pil operon reveal relationships between symbiotic strains of Vibrio fischeri . Int J Syst Evol Microbiol 58:1292–1299 [CrossRef]
    [Google Scholar]
  5. Cannone J. J., Subramanian S., Schnare M. N., Collett J. R., D'Souza L. M., Du Y., Feng B., Lin N., Madabusi L. V. other authors 2002; The comparative RNA web (CRW) site: an online database of comparative sequence and structure information for ribosomal, intron, and other RNAs. BMC Bioinformatics 3: 2 [CrossRef]
    [Google Scholar]
  6. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  8. El-Baradei G., Delacroix-Buchet A., Ogier J. C. 2007; Biodiversity of bacterial ecosystems in traditional Egyptian Domiati cheese. Appl Environ Microbiol 73:1248–1255 [CrossRef]
    [Google Scholar]
  9. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  10. Feurer C., Irlinger F., Spinnler H. E., Glaser P., Vallaeys T. 2004; Assessment of the rind microbial diversity in a farmhouse-produced vs a pasteurized industrially produced soft red-smear cheese using both cultivation and rDNA-based methods. J Appl Microbiol 97:546–556 [CrossRef]
    [Google Scholar]
  11. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  12. Kümmerle M., Scherer S., Seiler H. 1998; Rapid and reliable identification of food-borne yeasts by Fourier-transform infrared spectroscopy. Appl Environ Microbiol 64:2207–2214
    [Google Scholar]
  13. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. other authors 2007; clustal w and clustal_x version 2.0. Bioinformatics 23:2947–2948 [CrossRef]
    [Google Scholar]
  14. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  15. Martinez-Urtaza J., Lozano-Leon A., Vina-Feas A., de Novoa J., Garcia-Martin O. 2006; Differences in the API 20E biochemical patterns of clinical and environmental Vibrio parahaemolyticus isolates. FEMS Microbiol Lett 255:75–81 [CrossRef]
    [Google Scholar]
  16. Mellado E., Moore E. R., Nieto J. J., Ventosa A. 1996; Analysis of 16S rRNA gene sequences of Vibrio costicola strains: description of Salinivibrio costicola gen. nov., comb. nov. Int J Syst Bacteriol 46:817–821 [CrossRef]
    [Google Scholar]
  17. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479:297–306 [CrossRef]
    [Google Scholar]
  18. Mounier J., Gelsomino R., Goerges S., Vancanneyt M., Vandemeulebroecke K., Hoste B., Scherer S., Swings J., Fitzgerald G. F., Cogan T. M. 2005; Surface microflora of four smear-ripened cheeses. Appl Environ Microbiol 71:6489–6500 [CrossRef]
    [Google Scholar]
  19. Nam Y. D., Chang H. W., Park J. R., Kwon H. Y., Quan Z. X., Park Y. H., Kim B. C., Bae J. W. 2007; Vibrio litoralis sp. nov., isolated from a Yellow Sea tidal flat in Korea. Int J Syst Evol Microbiol 57:562–565 [CrossRef]
    [Google Scholar]
  20. Naumann D., Helm D., Labischinski H. 1991; Microbiological characterizations by FT-IR spectroscopy. Nature 351:81–82 [CrossRef]
    [Google Scholar]
  21. Thompson F. L., Iida T., Swings J. 2004; Biodiversity of vibrios. Microbiol Mol Biol Rev 68:403–431 [CrossRef]
    [Google Scholar]
  22. Thompson F. L., Gevers D., Thompson C. C., Dawyndt P., Naser S., Hoste B., Munn C. B., Swings J. 2005; Phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis. Appl Environ Microbiol 71:5107–5115 [CrossRef]
    [Google Scholar]
  23. Thompson C. C., Thompson F. L., Vicente A. C. P., Swings J. 2007; Phylogenetic analysis of vibrios and related species by means of atpA gene sequences. Int J Syst Evol Microbiol 57:2480–2484 [CrossRef]
    [Google Scholar]
  24. Van de Peer Y., De Wachter R. 1997; Construction of evolutionary distance trees with treecon for Windows: accounting for variation in nucleotide substitution rate among sites. Comput Appl Biosci 13:227–230
    [Google Scholar]
  25. Verbarg S., Frühling A., Cousin S., Brambilla E., Gronow S., Lünsdorf H., Stackebrandt E. 2008; Biostraticola tofi gen. nov., spec. nov., a novel member of the family Enterobacteriaceae . Curr Microbiol 56603–608 [CrossRef]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  27. Wenning M., Theilmann V., Scherer S. 2006; Rapid analysis of two food-borne microbial communities at the species level by Fourier-transform infrared microspectroscopy. Environ Microbiol 8:848–857 [CrossRef]
    [Google Scholar]
  28. Yumoto I. I., Iwata H., Sawabe T., Ueno K., Ichise N., Matsuyama H., Okuyama H., Kawasaki K. 1999; Characterization of a facultatively psychrophilic bacterium, Vibrio rumoiensis sp. nov., that exhibits high catalase activity. Appl Environ Microbiol 65:67–72
    [Google Scholar]
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