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Abstract

Strain DCY21, a Gram-negative, gliding and rod-shaped aerobic bacterium was isolated from soil of a ginseng field in the Republic of Korea and characterized using a polyphasic approach in order to determine its taxonomic position. Comparative 16S rRNA gene sequence analysis revealed that strain DCY21 clustered with the species of the genus . It was closely related to LMG 8763 (97.9 %), YC5194 (97.6 %), DSM 2044 (97.5 %), DSM 18481 (97.2 %) and DSM 2043 (96.9 %). The major cellular fatty acids of strain DCY21 were iso-C (34.3 %), iso-C 9 (19.5 %) and iso-C (17.2 %) and the major isoprenoid quinone was Q-8. The major polar lipids of strain DCY21 were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidyl--methylethanolamine. The G+C content of the total DNA was 65.4 mol%. The DNA–DNA relatedness values, and biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiation of strain DCY21 from species of the genus . Strain DCY21 therefore represents a novel species, for which the name sp. nov. is proposed. The type strain is DCY21 (=KCTC 22011 =LMG 24126).

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2010-07-01
2019-10-21
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Two-dimensional thin-layer chromatograms of polar lipids of strain DCY21 visualized with ninhydrin (for amino group) and 5% ethanolic molybdatophosphoric acid (for total lipids). PE, Phosphatidylethanolamine; PG, phosphatidylglycerol; DPG, diphosphatidylglycerol; L1, unknown glycolipid; AL1 unknown amino lipid; PME, phosphatidyl-N-methylethanolamine.

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