@article{mbs:/content/journal/ijsem/10.1099/ijs.0.016402-0, author = "Satoh, Kazuo and Ooe, Kenji and Nagayama, Hirotoshi and Makimura, Koichi", title = "Prototheca cutis sp. nov., a newly discovered pathogen of protothecosis isolated from inflamed human skin", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2010", volume = "60", number = "5", pages = "1236-1240", doi = "https://doi.org/10.1099/ijs.0.016402-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.016402-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A strain of a novel pathogenic, achlorophyllic alga belonging to the genus Prototheca was isolated from the inflamed skin of a patient with protothecosis in a Japanese hospital. The pathogen was detected and isolated in biopsy specimens by histopathology and culture-based examination. Analyses of the nuclear 18S rDNA gene and 26S rDNA gene D1/D2 domain sequences and chemotaxonomic studies indicated that this strain represents a novel species with a close phylogenetic relationship to Prototheca wickerhamii and Auxenochlorella protothecoides. This strain grew well at 28–30 °C, showed slow and weak growth at 37 °C, and no growth at 40 °C. This strain grew in vitamin-free medium and assimilated acetate (pH 5.1), l-arabinose and soluble starch as a carbon source. The taxonomic description of Prototheca cutis sp. nov. is proposed (type strain JCM 15793T =CBS 11262T =DSM 22084T) as a pathogen of dermatitis.", }