1887

Abstract

A strain of a novel pathogenic, achlorophyllic alga belonging to the genus was isolated from the inflamed skin of a patient with protothecosis in a Japanese hospital. The pathogen was detected and isolated in biopsy specimens by histopathology and culture-based examination. Analyses of the nuclear 18S rDNA gene and 26S rDNA gene D1/D2 domain sequences and chemotaxonomic studies indicated that this strain represents a novel species with a close phylogenetic relationship to and This strain grew well at 28–30 °C, showed slow and weak growth at 37 °C, and no growth at 40 °C. This strain grew in vitamin-free medium and assimilated acetate (pH 5.1), -arabinose and soluble starch as a carbon source. The taxonomic description of sp. nov. is proposed (type strain JCM 15793 =CBS 11262 =DSM 22084) as a pathogen of dermatitis.

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2010-05-01
2020-01-25
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The symptom that was caused by infection with strain JCM 15793 . Cellulitis-like dermatitis with ulcer (70×25 mm) was observed on the left forearm.

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Histopathological image of biopsy specimen. Bar, 10 µm.

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Phylogenetic tree drawn from neighbour-joining analysis of 26S rDNA gene D1/D2 domain sequences, depicting the relationships of strain JCM 15793 with closely related species. Bootstrap percentages over 60% from 1000 bootstrap replicates are shown. Bar, 0.05 substitutions per nucleotide position. Outgroup: CC-1952 (AF183463).

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