1887

Abstract

Morphological and chemotaxonomic characterization of actinomycete strain MW2-25, isolated from tropical forest soil in Nakhon Sawan Province, Thailand, clearly demonstrated that this strain belongs to the genus . Phylogenetic analysis using 16S rRNA gene sequences also indicated that this strain should be classified in the genus and showed that the closest relative was IFO 12592 (99.3 % sequence similarity). DNA–DNA hybridization values and some physiological and biochemical properties indicated that this strain could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name sp. nov. is proposed. The type strain is MW2-25 (=BCC 34832=JCM 15933).

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2010-05-01
2024-04-18
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References

  1. Arai T. 1975 Culture Media for Actinomycetes Tokyo: The Society for Actinomycetes Japan;
    [Google Scholar]
  2. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  7. Hamaki T., Suzuki M., Fudou R., Jojima Y., Kajiura T., Tabuchi A., Sen K., Shibai H. 2005; Isolation of novel bacteria and actinomycetes using soil-extract agar medium. J Biosci Bioeng 99:485–492 [CrossRef]
    [Google Scholar]
  8. Hayakawa M., Sadakata T., Kajiura T., Nonomura H. 1991; New methods for the highly selective isolation of Micromonospora and Microbispora from soil. J Ferment Bioeng 72:320–326 [CrossRef]
    [Google Scholar]
  9. Itoh T., Kudo T., Parenti F., Seino A. 1989; Amended description of the genus Kineosporia , based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 39:168–173 [CrossRef]
    [Google Scholar]
  10. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  11. Kawamoto I., Oka T., Nara T. 1981; Cell wall composition of Micromonospora olivoasterospora , Micromonospora sagamiensis , and related organisms. J Bacteriol 146:527–534
    [Google Scholar]
  12. Kluge A. G., Farris J. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  13. Koch C., Kroppenstedt R. M., Rainey F. A., Stackebrandt E. 1996; 16S ribosomal DNA analysis of the genera Micromonospora ,Actinoplanes , Catellatospora , Catenuloplanes , Couchioplanes , Dactylosporangium, and Pilimelia and emendation of the family Micromonosporaceae . Int J Syst Bacteriol 46:765–768 [CrossRef]
    [Google Scholar]
  14. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  15. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20) pp 173–199 Edited by Goodfellow M., Minnikin D. E. New York: Academic Press;
    [Google Scholar]
  16. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: Molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  17. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  18. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy pp 227–291 Edited by Dietz A., Thayer D. W. Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  19. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  20. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium , Nocardia , and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204 [CrossRef]
    [Google Scholar]
  21. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  23. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  24. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  25. Shomura T., Kojima M., Yoshida J., Ito M., Amano S., Totsugawa K., Niwa T., Inouye S., Ito T., Niida T. 1980; Studies on a new aminoglycoside antibiotic, dactimicin. I. Producing organism and fermentation. J Antibiot 33:924–930 [CrossRef]
    [Google Scholar]
  26. Shomura T., Yoshida J., Miyadoh S., Ito T., Niida T. 1983; Dactylosporangium vinaceum sp. nov. Int J Syst Bacteriol 33:309–313 [CrossRef]
    [Google Scholar]
  27. Shomura T., Amano S., Tohyama H., Yoshida J., Ito T., Niida T. 1985; Dactylosporangium roseum sp. nov. Int J Syst Bacteriol 35:1–4 [CrossRef]
    [Google Scholar]
  28. Shomura T., Amano S., Yoshida J., Kojima M. 1986; Dactylosporangium fulvum sp. nov. Int J Syst Bacteriol 36:166–169 [CrossRef]
    [Google Scholar]
  29. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  30. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  31. Suriyachadkun C., Chunhametha S., Thawai C., Tamura T., Potacharoen W., Kirtikara K., Sanglier J. J. 2009; Planotetraspora thailandica sp. nov., isolated from soil in Thailand. Int J Syst Evol Microbiol 59:992–997 [CrossRef]
    [Google Scholar]
  32. Tamaoka J. 1994; Determination of DNA base composition. In Chemical Methods in Prokaryotic Systematics pp 463–470 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  33. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  34. Thiemann J. E. 1970; Dactylosporangium thailandensis should be D. thailandense . Int J Syst Bacteriol 20:59 [CrossRef]
    [Google Scholar]
  35. Thiemann J. E., Pagani H., Beretta G. 1967; A new genus of the Actinoplanaceae : Dactylosporangium , gen. nov. Arch Mikrobiol 58:42–52 [CrossRef]
    [Google Scholar]
  36. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  37. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [CrossRef]
    [Google Scholar]
  38. Verlander C. P. 1992; Detection of horseradish peroxidase by colorimetry. In Nonisotopic DNA Probe Techniques pp 185–201 Edited by Kricka L. J. New York: Academic Press;
    [Google Scholar]
  39. Williams S. T., Cross T. 1971; Actinomycetes. Methods Microbiol 4:295–334
    [Google Scholar]
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