Two freshwater isolates (WB4.1-19T and WB4.4-101), sharing 99.9 % 16S rRNA gene sequence similarity, were highly related to Aeromonas sobria (99.7 % similarity; 6 bp differences). A phylogenetic tree derived from a multi-locus phylogenetic analysis (MLPA) of the concatenated sequences of five housekeeping genes (gyrB, rpoD, recA, dnaJ and gyrA; 3684 bp) revealed that both strains clustered as an independent phylogenetic line next to members of Aeromonas molluscorum and Aeromonas bivalvium. The DNA–DNA reassociation value between the two new isolates was 89.3 %. Strain WB4.1-19T had a DNA–DNA relatedness value of <70 % with the type strains of the other species tested. Phenotypic characterization differentiated the two novel strains from all other type strains of species of the genus Aeromonas. It is concluded that the two new strains represent a novel species of the genus Aeromonas, for which the name Aeromonas rivuli sp. nov. is proposed, with the type strain WB4.1-19T (=CECT 7518T=DSM 22539T=MDC 2511T).
AlperiA.,
FiguerasM. J.,
InzaI.,
Martínez-MurciaA. J.2008; Analysis of 16S rRNA gene mutations in a subset of Aeromonas strains and their impact in species delineation. Int Microbiol 11:185–194
BrosiusJ.,
PalmerM. L.,
KennedyP. J.,
NollerH. F.1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli
. Proc Natl Acad Sci U S A 75:4801–4805[CrossRef]
CousinS.,
BrambillaE.,
YangJ.,
StackebrandtE.2008; Culturable aerobic bacteria from the upstream region of a karst water rivulet. Int Microbiol 11:91–100
HuysG.,
KämpferP.,
AltweggM.,
KerstersI.,
LambA.,
CoopmanR.,
Lüthy-HottensteinJ.,
VancanneytM.,
JanssenP.,
KerstersK.1997; Aeromonas popoffii sp. nov., a mesophilic bacterium isolated from drinking water production plants and reservoirs. Int J Syst Bacteriol 47:1165–1171[CrossRef]
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120[CrossRef]
Martin-CarnahanA.,
JosephS. W.2005; Order XII. Aeromonadales ord. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn. vol 2Bp–556 Edited by
BrennerD. J.,
KriegN. R.,
StaleyJ. T.,
GarrityG. M.
New York: Springer;
Martínez-MurciaA. J.,
BenllochS.,
CollinsM. D.1992; Phylogenetic interrelationships of members of the genera Aeromonas and Plesiomonas as determined by 16S ribosomal DNA sequencing: lack of congruence with results of DNA-DNA hybridizations. Int J Syst Bacteriol 42:412–421[CrossRef]
Miñana-GalbisD.,
Urbizu-SerranoA.,
FarfánM.,
FustéM. C.,
LorénG.2009; Phylogenetic analysis and identification of Aeromonas species based on sequencing of the cpn60 universal target. Int J Syst Evol Microbiol 59:1976–1983[CrossRef]
Miñana-GalbisD.,
FarfánM.,
Gaspar LorénJ.,
FustéM. C.2010; Proposal to assign Aeromonas diversa sp. nov. as a novel species designation for Aeromonas group 501. Syst Appl Microbiol 33:15–19[CrossRef]
NhungP. H.,
HataH.,
OhkusuK.,
NodaM.,
ShahM. M.,
GotoK.,
EzakiT.2007; Use of the novel phylogenetic marker dnaJ and DNA–DNA hybridization to clarify interrelationships within the genus Aeromonas
. Int J Syst Evol Microbiol 57:1232–1237[CrossRef]
ØrmenO.,
GranumP. E.,
LassenJ.,
FiguerasM. J.2005; Lack of agreement between biochemical and genetic identification of Aeromonas spp. APMIS 113:203–207[CrossRef]
SepeA.,
BarbieriP.,
PeduzziR.,
DemartaA.2008; Evaluation of recA sequencing for the classification of Aeromonas strains at the genotype level. Lett Appl Microbiol 46:439–444[CrossRef]
SolerL.,
YañezM. A.,
ChacónM. R.,
Aguilera-ArreolaM. G.,
CatalánV.,
FiguerasM. J.,
Martínez-MurciaA. J.2004; Phylogenetic analysis of the genus Aeromonas based on two housekeeping genes. Int J Syst Evol Microbiol 54:1511–1519[CrossRef]
StackebrandtE.,
FrederiksenW.,
GarrityG. M.,
GrimontA. D.,
KämpferP.,
MaidenM. C. J.,
NesmeX.,
Roselló-MoraR.,
SwingsJ.other authors2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047[CrossRef]
ThompsonJ. D.,
HigginsD. G.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
YáñezM. A.,
CatalánV.,
ApraizD.,
FiguerasM. J.,
Martínez-MurciaA. J.2003; Phylogenetic analysis of members of the genus Aeromonas based on gyrB gene sequences. Int J Syst Evol Microbiol 53:875–883[CrossRef]