1887

Abstract

Two strains of pink-coloured bacteria, 5516T-9 and 5516T-11, were isolated from an air sample collected in Korea. The taxonomic status of these novel strains was investigated by means of a polyphasic approach. The novel strains were Gram-positive, aerobic, non-spore-forming and coccus-shaped bacteria. The DNA G+C contents of strains 5516T-9 and 5516T-11 were 61.0 and 59.3 mol%, respectively. The major isoprenoid quinone for both strains was MK-8. Strain 5516T-9 contained summed feature 3 (iso-C 2-OH and/or C 7), C and iso-C 9, and strain 5516T-11 contained summed feature 3, iso-C 9, C 8 and C 6 as the major fatty acids (>10 %). The polar lipid patterns of both strains were similar, comprising one phospholipid and one aminophospholipid as the major components. Phylogenetic analyses using 16S rRNA gene sequences showed that both novel strains were affiliated to the genus . Strain 5516T-9 exhibited the highest sequence similarity with DSM 12784 (96.8 %) and strain 5516T-11 showed the highest sequence similarity with DSM 15974 (94.5 %). The sequence similarity between strains 5516T-9 and 5516T-11 was 94.7 %. On the basis of the data presented, it is evident that both strains represent separate novel species of the genus for which the names sp. nov. (type strain 5516T-9=KACC 12745=JCM 15442) and sp. nov. (type strain 5516T-11=KACC 12746=JCM 15443) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.016030-0
2010-05-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/5/1191.html?itemId=/content/journal/ijsem/10.1099/ijs.0.016030-0&mimeType=html&fmt=ahah

References

  1. Breznak, J. A. & Costilow, R. N. ( 1994; ). Physicochemical factors in growth. In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  2. Callegan, R. P., Nobre, M. F., McTernan, P. M., Battista, J. R., Navarro-Gonzalez, R., McKay, C. P., da Costa, M. S. & Rainey, F. A. ( 2008; ). Description of four novel psychrophilic, ionizing radiation-sensitive Deinococcus species from alpine environments. Int J Syst Evol Microbiol 58, 1252–1258.[CrossRef]
    [Google Scholar]
  3. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  4. Fitch, W. M. ( 1971; ). Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  5. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  6. Hirsch, P., Gallikowski, C. A., Siebert, J., Peissl, K., Kroppenstedt, R. M., Schumann, P., Stackebrandt, E. & Anderson, R. ( 2004; ). Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental Antarctica. Syst Appl Microbiol 27, 636–645.[CrossRef]
    [Google Scholar]
  7. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  8. Kwon, S. W., Kim, J. S., Park, I. C., Yoon, S. H., Park, D. H., Lim, C. K. & Go, S. J. ( 2003; ). Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53, 21–27.[CrossRef]
    [Google Scholar]
  9. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  10. Peng, F., Zhang, L., Luo, X., Dai, J., An, H., Tang, Y. & Fang, C. ( 2009; ). Deinococcus xinjiangensis sp. nov., isolated from desert soil. Int J Syst Evol Microbiol 59, 709–713.[CrossRef]
    [Google Scholar]
  11. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  12. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  13. Weon, H. Y., Kim, B. Y., Schumann, P., Son, J. A., Jang, J., Go, S. J. & Kwon, S. W. ( 2007; ). Deinococcus cellulosilyticus sp. nov., isolated from air. Int J Syst Evol Microbiol 57, 1685–1688.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.016030-0
Loading
/content/journal/ijsem/10.1099/ijs.0.016030-0
Loading

Data & Media loading...

Two-dimensional TLC of polar lipid extracts obtained from strains 5516T-9 (a) and 5516T-11 (b) sprayed with anisaldehyde. L1–L12, Unidentified polar lipids; PL, unidentified phospholipid; APL1–APL2, unidentified aminophospholipids.

IMAGE

Two-dimensional TLC of polar lipid extracts obtained from strains 5516T-9 (a) and 5516T-11 (b) sprayed with anisaldehyde. L1–L12, Unidentified polar lipids; PL, unidentified phospholipid; APL1–APL2, unidentified aminophospholipids.

IMAGE

Maximum-parsimony phylogenetic tree, based on 16S rRNA gene sequences, showing the positions of strains 5516T-9 and 5516T-11 and species of the genus . [ PDF] 51 KB

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error