1887

Abstract

A Gram-negative, non-pigmented, non-motile, chemoheterotrophic marine bacterium, designated strain IMCC2782, was isolated from a surface seawater sample of the Yellow Sea, Korea. On the basis of 16S rRNA gene sequence analysis and phenotypic and genotypic characterization, strain IMCC2782 is shown to represent a novel species of the genus . Strain IMCC2782 grew at 15–37 °C and tolerated 7.5 % NaCl. Based on 16S rRNA gene sequences, strain IMCC2782 was related most closely to the type strain of (97.1 % similarity) but showed less than 90 % similarity to the type strains of other bacterial species. The predominant fatty acids were mono-unsaturated C and C. The G+C content of the DNA of strain IMCC2782 was 59.6 mol%. A low level of DNA–DNA relatedness (28.1 %) together with several phenotypic characteristics, including enzyme activities, served to differentiate strain IMCC2782 from the type strain of . Therefore, strain IMCC2782 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IMCC2782 (=KCTC 22683 =NBRC 105824).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.016022-0
2010-06-01
2020-01-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/6/1303.html?itemId=/content/journal/ijsem/10.1099/ijs.0.016022-0&mimeType=html&fmt=ahah

References

  1. Cho, J.-C. & Giovannoni, S. J. ( 2003; ). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria. Int J Syst Evol Microbiol 53, 1031–1036.[CrossRef]
    [Google Scholar]
  2. Choo, Y.-J., Lee, K., Song, J. & Cho, J.-C. ( 2007; ). Puniceicoccus vermicola gen. nov., sp. nov., a novel marine bacterium, and description of Puniceicoccaceae fam. nov., Puniceicoccales ord. nov., Opitutaceae fam. nov., Opitutales ord. nov. and Opitutae classis nov. in the phylum ‘Verrucomicrobia’. Int J Syst Evol Microbiol 57, 532–537.[CrossRef]
    [Google Scholar]
  3. Chun, J., Lee, J. H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  6. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  7. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  8. Kim, H., Choo, Y.-J. & Cho, J.-C. ( 2007; ). Litoricolaceae fam. nov., to include Litoricola lipolytica gen. nov., sp. nov., a marine bacterium belonging to the order Oceanospirillales. Int J Syst Evol Microbiol 57, 1793–1798.[CrossRef]
    [Google Scholar]
  9. Kovacs, N. ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178, 703
    [Google Scholar]
  10. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  11. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  12. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  13. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
    [Google Scholar]
  14. Swofford, D. L. ( 2002; ). paup*: phylogenetic analysis using parsimony (and other methods), version 4.0 Beta. Sunderland, MA: Sinauer Associates.
  15. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.016022-0
Loading
/content/journal/ijsem/10.1099/ijs.0.016022-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error