1887

Abstract

During an investigation of the microbiology of Ghanaian cocoa fermentations, a number of yeast isolates with unusual pheno- and genotypic properties representing three possible novel species were isolated. Members of Group A divided by multilateral budding and ascospores were not produced. Group B strains produced true hyphae and ascospores were not produced. Group C representatives divided by budding and formed chains and star-like aggregates. Ascospores were not produced. Sequence analysis of the 26S rRNA gene (D1/D2 region) revealed that the Group A isolates were phylogenetically most closely related to (gene sequence similarity 92.4 %), (88.8 %), (88.8 %) and (88.3 %). Members of Group B were most closely related to representatives of the genera and and the asporogenous genus , but in all cases with 26S rRNA gene (D1/D2 region) similarities below 87 %. For Group C, the most closely related species were (92.4 %), (91.6 %) and (91.9 %). The very low gene sequence similarities obtained for the three groups of isolates clearly indicated that they represented novel species. Repetitive Palindromic PCR (Rep-PCR) of the isolates and their closest phylogenetic relatives confirmed that the new isolates belonged to previously undescribed species. In conclusion, based on the genetic and phenotypic results, the new isolates were considered to represent three novel species, for which the names (group A, type strain G3=CBS 11009=CCUG 56721); (group B, type strain G6=CBS 11010=CCUG 56722) and (group C, type strain G15=CBS 11011=CCUG 56723) are proposed.

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2010-06-01
2019-10-21
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vol. , part 6, pp. 1460 - 1465

(GTG)5-PCR fingerprints and corresponding dendrogram, derived from UPGMA linkage of correlation coefficients of isolate G3 and phylogenetic closest relatives.

(GTG)5-PCR fingerprints and corresponding dendrogram, derived from UPGMA linkage of correlation coefficients of isolate G6 , G17 and phylogenetic closest relatives.

(GTG)5-PCR fingerprints and corresponding dendrogram, derived from UPGMA linkage of correlation coefficients of isolate G15 , G68, G71, G174 and phylogenetic closest relatives.

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