1887

Abstract

A non-lipophilic coryneform bacterium isolated from an anaerobic Bactec bottle inoculated with an ankle aspirate from a male patient was characterized by phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of short-chain mycolic acids in the cell wall of the bacterium, a feature consistent with members of the genus . Comparative 16S rRNA gene sequence analysis demonstrated that the isolate displayed 92.0–99.0 % gene sequence similarity with members of the genus , with as the most closely related phylogenetic species (99.0 % gene sequence similarity). However, the isolate could be genomically separated from on the basis of DNA–DNA hybridization studies (39.5 % relatedness). Furthermore, the isolate could also be differentiated from and other species of the genus on the basis of biochemical properties. Based on both phenotypic and phylogenetic evidence, it is proposed that this isolate be classified as representing a novel species, sp. nov. (type strain IMMIB WACC 658=DSM 45350=CCUG 57942).

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2010-07-01
2019-10-14
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