1887

Abstract

Strain FhSp1, isolated from human blood in Spain in 2003, was studied for its taxonomic allocation. By 16S rRNA and gene sequencing, the strain was shown to belong to the genus . In the 16S rRNA gene sequence, sp. FhSp1 shared similarity of more than 99 % with strains of subspecies and U112, 98 % with GM2212 and 98.4 % with ATCC 25015. In the gene sequence, sp. FhSp1 exhibited 91.6–91.7 % similarity to strains of subspecies, 91.2 % to U112 and 84 % to ATCC 25017. The genus affiliation was supported by a quinone system typical of (Q-8 as the major component), a complex polar lipid profile similar to that of with the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unknown aminophospholipid (APL4) and a fatty acid profile consisting mainly of C (17.2 %), C (11.2 %), C (13.1 %), C 3-OH (14.2 %) and C 9 (7.1 %). DNA–DNA hybridization, which showed unambiguously that FhSp1 represents a novel species, and the results of biochemical tests allowed genotypic and phenotypic differentiation of the isolate from all hitherto-described species. A multiplex PCR developed in the course of this study discriminated FhSp1 from representatives of all other species and subspecies, clades A.I and A.II of subsp. and subsp. biovar japonica and also between these representatives of the genus. Therefore, we propose the name sp. nov., with the type strain FhSp1 (=FnSp1 =FSC454 =F62 =DSM 22475 =CCUG 58020). Furthermore, we formally propose the transfer of the species to the species as subsp. comb. nov. (type strain ATCC 15482 =CCUG 33449 =CIP 56.12). We also present an emended description of the genus .

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2010-08-01
2019-10-20
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