1887

Abstract

Two extremely halophilic archaea, strains RO1-28 and RO1-22, were isolated from a marine solar saltern in Jiangsu, China. Both strains required at least 0.05 M Mg and 1.7 M NaCl for growth. They were able to grow over a pH range of 6.0–8.5 and a temperature range of 25–55 °C, with optimal pH of 7.0 and optimal temperature of 37–40 °C. Based on 16S rRNA gene sequence analysis, strains RO1-28 and RO1-22 were closely related to , the single species of the genus , with similarities of 94.0–95.2 %. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and three glycolipids chromatographically identical to the glycolipids of JCM 13897. Both strains RO1-28 and RO1-22 had a DNA G+C content of 54.0 mol% (HPLC). The DNA–DNA hybridization value between the two strains was more than 70 % (92 %) and both strains showed low levels of DNA–DNA relatedness (32 % and 33 %) with JCM 13897. It was concluded that strains RO1-28 and RO1-22 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RO1-28 (=CGMCC 1.7737 =JCM 15771).

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2010-05-01
2020-09-26
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