1887

Abstract

The taxonomic positions of two gamma- and UV-ray-resistant strains isolated from radiation-polluted soil in north-west China were determined in a polyphasic study. The organisms, designated R12 and R13, were Gram-stain-positive, non-spore-forming cocci, which contained MK-8 as the major respiratory quinone and C 7 and C as major fatty acids. The cell walls of strains R12 and R13 contained ornithine. Phylogenetic analysis based on 16S rRNA gene sequences and DNA–DNA hybridizations showed that strains R12 and R13 are members of novel species belonging to the genus with DSM 20539 as the closest relative. The isolates R12 and R13 shared 97 and 97.1 % 16S rRNA gene similarity, respectively, and 29.5 and 33.3 % DNA–DNA relatedness, respectively, with DSM 20539. The DNA G+C contents of isolates R12 and R13 were 66.7 and 63.8 %, respectively. On the basis of phenotypic tests and other results, two species, sp. nov. (type strain R12 =CGMCC 1.8884 =NBRC 105665) and sp. nov. (type strain R13 =CGMCC 1.8885 =NBRC 105666), are proposed.

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2010-09-01
2019-10-20
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vol. , part 9, pp. 2006 - 2010

Two-dimensional thin-layer chromatography of polar lipids of R1 , strain R12 and strain R13 . Components were visualized by staining with 5% molybdophosphoric acid in ethanol followed by heating of the plates at 130°C. PGL, unidentified phosphoglycolipid; GL, unidentified glycolipid; PL, unidentified phospholipids; APL, unidentified aminophospholipid; PIG, pigment.

Fatty acid compositions of strains R12 and R13

Data are percentages of total fatty acids.



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