A xylanolytic bacterium, strain S1-3T, was isolated from soil collected in Nan province, Thailand. It was characterized taxonomically based on phenotypic characteristics and 16S rRNA gene sequence comparison. The strain was a Gram-stain-positive, facultatively anaerobic, spore-forming, rod-shaped bacterium. It contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major menaquinone was MK-7. Iso-C16 : 0 (39.5 %) and anteiso-C15 : 0 (26.8 %) were predominant cellular fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol were the major polar lipids. The DNA G+C content was 53.3 mol%. Phylogenetic analysis using 16S rRNA gene sequences showed that strain S1-3T was affiliated to the genus Cohnella, and was closely related to Cohnella ginsengisoli GR21-5T and Cohnella thermotolerans CCUG 47242T with 95.7 and 95.3 % sequence similarity, respectively. Strain S1-3T could be clearly distinguished from related species of the genus Cohnella by its physiological and biochemical characteristics as well as by its phylogenetic position. Therefore, the strain represents a novel species of the genus Cohnella, for which the name Cohnella thailandensis sp. nov. is proposed. The type strain is S1-3T (=KCTC 22296T =TISTR 1890T =PCU 306T).
García-FraileP.,
VelázquezE.,
MateosP. F.,
Martínez-MolinaE.,
RivasR.2008; Cohnella phaseoli sp. nov., isolated from root nodules of Phaseolus coccineus in Spain, and emended description of the genus Cohnella
. Int J Syst Evol Microbiol 58:1855–1859[CrossRef]
MinnikinD. E.,
PatelP. V.,
AlshamaonyL.,
GoodfellowM.1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117[CrossRef]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128[CrossRef]
TeatherR. M.,
WoodP. J.1982; Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43:777–780
TengJ. L. L.,
WooP. C. Y.,
LeungK. W.,
LauS. K. P.,
WongM. K. M.,
YuenK. Y.2003; Pseudobacteraemia in a patient with neutropenic fever caused by a novel paenibacillus species: Paenibacillus hongkongensis sp. nov. Mol Pathol 56:29–35[CrossRef]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]